Simultaneous determination of 3,3′,4′,5,7-pentamethylquercetin and its possible metabolite 3,3′,4′,7-tetramethylquercetin in dog plasma by liquid chromatography-tandem mass spectrometry and its application to preclinical pharmacokinetic study

被引:7
作者
Chen, Xiaomei [1 ]
Li, Dongyan [1 ]
Hu, Yan [2 ]
Jin, Manwen [2 ]
Zhou, Liping [1 ]
Peng, Kelong [1 ]
Zheng, Heng [1 ]
机构
[1] Huazhong Univ Sci & Technol, Dept Pharm, Tongji Hosp, Tongji Med Coll, Wuhan 430030, Peoples R China
[2] Huazhong Univ Sci & Technol, Dept Pharmacol, Sch Basic Med Sci, Tongji Med Coll, Wuhan 430030, Peoples R China
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2011年 / 879卷 / 23期
基金
中国国家自然科学基金;
关键词
3,3 ',4 ',5,7-pentamethylquercetin; 3,3 ',4 ',7-tetramethylquercetin; LC-MS/MS; Pharmacokinetic; CARDIOVASCULAR-DISEASE; FLAVONOIDS; QUERCETIN; APOPTOSIS; CELLS; RISK;
D O I
10.1016/j.jchromb.2011.06.019
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive, simple and rapid ultra fast liquid chromatography (UFLC)-ESI-MS/MS method was established for the simultaneous determination of 3,3',4',5,7-pentamethylquercetin (PMQ) and its possible metabolite 3,3',4',7-tetramethylquercetin (TMQ) in dog plasma using 4',5,7-trimethylapigenin (TMA) as the internal standard. The plasma sample was pretreated with acetonitrile for protein precipitation and the analytes were separated on an Ultimate XB-CN column (5 mu m, 2.1 mm x 150 mm) with the mobile phase consisting of acetonitrile and water (2:1, v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer under a positive multiple reaction-monitoring mode (MRM). The mass transition ion-pair was followed as m/z 373.1-312.1 for PMQ 359.1-344.0 for TMQ and 313.1-298.1 for TMA. The validated concentration ranged from 1.272 to 3060 ng/mL for PMQ and from 10.35 to 1725 ng/mL for TMQ. The lower limit of quantifications for PMQ and TMQ were 1.272 ng/mL and 10.35 ng/mL, respectively. The developed-method was successfully applied for the pharmacokinetic study of PMQ and its metabolite TMQ in dogs following a single oral dose. (C) 2011 Elsevier BY. All rights reserved.
引用
收藏
页码:2339 / 2344
页数:6
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