Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy

被引：24

作者：

Clendenon, Sherry G. ^{[1
]}

Young, Pamela A. ^{[1
]}

Ferkowicz, Michael ^{[2
,3
]}

Phillips, Carrie ^{[4
,5
]}

Dunn, Kenneth W. ^{[1
]}

机构：

[1] Indiana Univ, Med Ctr, Dept Med, Div Nephrol, Indianapolis, IN 46202 USA

[2] Indiana Univ Sch Med, Herman B Wells Ctr Pediat Res, Indianapolis, IN 46202 USA

[3] Indiana Univ Sch Med, Dept Pediat, Indianapolis, IN 46202 USA

[4] Indiana Univ Sch Med, Div Nephrol, Indianapolis, IN 46202 USA

[5] Indiana Univ Sch Med, Dept Pathol, Indianapolis, IN 46202 USA

来源：

MICROSCOPY AND MICROANALYSIS | 2011年 / 17卷 / 04期

基金：

美国国家卫生研究院;

关键词：

confocal; multiphoton; clearing; refractive index matching; scattering; 2-PHOTON MICROSCOPY; RESOLUTION;

D O I：

10.1017/S1431927611000535

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or multiphoton microscopy. Tissue clearing appears to eliminate the need for multiphoton excitation for deep tissue imaging.

引用

页码：614 / 617

页数：4

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