Direct multiplexed measurement of gene expression with color-coded probe pairs

被引：1603

作者：

Geiss, Gary K. ^{[1
]}

Bumgarner, Roger E. ^{[2
]}

Birditt, Brian ^{[1
]}

Dahl, Timothy ^{[1
]}

Dowidar, Naeem ^{[1
]}

Dunaway, Dwayne L. ^{[1
]}

Fell, H. Perry ^{[1
]}

Ferree, Sean ^{[1
]}

George, Renee D. ^{[1
]}

Grogan, Tammy ^{[1
]}

James, Jeffrey J. ^{[1
]}

Maysuria, Malini ^{[1
]}

Mitton, Jeffrey D. ^{[1
]}

Oliveri, Paola ^{[3
]}

Osborn, Jennifer L. ^{[1
]}

Peng, Tao ^{[2
]}

Ratcliffe, Amber L. ^{[1
]}

Webster, Philippa J. ^{[1
]}

Davidson, Eric H. ^{[3
]}

Hood, Leroy ^{[4
]}

机构：

[1] NanoString Technol Inc, Seattle, WA 98119 USA

[2] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA

[3] CALTECH, Div Biol 156 29, Pasadena, CA 91125 USA

[4] Inst Syst Biol, Seattle, WA 98103 USA

来源：

NATURE BIOTECHNOLOGY | 2008年 / 26卷 / 03期

关键词：

D O I：

10.1038/nbt1385

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a technology, the NanoString nCounter gene expression system, which captures and counts individual mRNA transcripts. Advantages over existing platforms include direct measurement of mRNA expression levels without enzymatic reactions or bias, sensitivity coupled with high multiplex capability, and digital readout. Experiments performed on 509 human genes yielded a replicate correlation coefficient of 0.999, a detection limit between 0.1 fM and 0.5 fM, and a linear dynamic range of over 500-fold. Comparison of the NanoString nCounter gene expression system with microarrays and TaqMan PCR demonstrated that the nCounter system is more sensitive than microarrays and similar in sensitivity to real-time PCR. Finally, a comparison of transcript levels for 21 genes across seven samples measured by the nCounter system and SYBR Green real-time PCR demonstrated similar patterns of gene expression at all transcript levels.

引用

页码：317 / 325

页数：9

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