Stem rust resistance in wheat is suppressed by a subunit of the mediator complex

被引：42

作者：

Hiebert, Colin W. ^{[1
]}

Moscou, Matthew J. ^{[2
]}

Hewitt, Tim ^{[3
,4
]}

Steuernagel, Burkhard ^{[5
]}

Hernandez-Pinzon, Inma ^{[2
]}

Green, Phon ^{[2
]}

Pujol, Vincent ^{[6
]}

Zhang, Peng ^{[3
]}

Rouse, Matthew N. ^{[7
]}

Jin, Yue ^{[7
]}

McIntosh, Robert A. ^{[3
]}

Upadhyaya, Narayana ^{[4
]}

Zhang, Jianping ^{[4
]}

Bhavani, Sridhar ^{[8
]}

Vrana, Jan ^{[9
]}

Karafiatova, Miroslava ^{[9
]}

Huang, Li ^{[10
]}

Fetch, Tom ^{[1
]}

Dolezel, Jaroslav ^{[9
]}

Wulff, Brande B. H. ^{[5
]}

Lagudah, Evans ^{[4
]}

Spielmeyer, Wolfgang ^{[4
]}

机构：

[1] Agr & Agri Food Canada, Morden Res & Dev Ctr, 101 Route 100, Morden, MB R6M 1Y5, Canada

[2] Univ East Anglia, Sainsbury Lab, Norwich Res Pk, Norwich NR4 7UK, Norfolk, England

[3] Univ Sydney, Plant Breeding Inst Cobbitty, Private Bag 4011, Narellan, NSW 2567, Australia

[4] CSIRO Agr & Food, GPO Box 1700, Canberra, ACT 2601, Australia

[5] John Innes Ctr, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

[6] Australian Natl Univ, Res Sch Biol, Acton, ACT 2601, Australia

[7] Univ Minnesota, ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA

[8] CIMMYT, ICRAF House,United Nations Ave, Nairobi 00621, Kenya

[9] Czech Acad Sci, Inst Expt Bot, Ctr Reg Hana Biotechnol & Agr Res, Slechtitelu 31, Olomouc 77900, Czech Republic

[10] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA

来源：

NATURE COMMUNICATIONS | 2020年 / 11卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 美国国家科学基金会;

关键词：

F-SP TRITICI; AEGILOPS-TAUSCHII COSS; DEFENSE GENE-EXPRESSION; LEAF RUST; HEXAPLOID WHEAT; COMMON WHEAT; PHYLOGENETIC ANALYSIS; STRIPE RUST; AESTIVUM L; RACE TTKS;

D O I：

10.1038/s41467-020-14937-2

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat. Stem rust is an important disease of wheat and resistance present in some cultivars can be suppressed by the SuSr-D1 locus. Here the authors show that SuSr-D1 encodes a subunit of the Mediator Complex and that nonsense mutations are sufficient to abolish suppression and confer stem rust resistance.

引用

页数：10

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