Analysis of the paracrine loop between cancer cells and fibroblasts using a microfluidic chip

被引：42

作者：

Hsu, Tsi-Hsuan ^{[1
]}

Xiao, Jian-Long ^{[1
,2
]}

Tsao, Yu-Wei ^{[2
,3
]}

Kao, Yi-Lun ^{[3
]}

Huang, Shih-Hao ^{[3
]}

Liao, Wei-Yu ^{[4
,5
]}

Lee, Chau-Hwang ^{[1
,2
,6
]}

机构：

[1] Natl Yang Ming Univ, Inst Biophoton, Taipei 11221, Taiwan

[2] Acad Sinica, Res Ctr Appl Sci, Taipei 11529, Taiwan

[3] Natl Taiwan Ocean Univ, Dept Mech & Mechatron Engn, Keelung 20224, Taiwan

[4] Natl Taiwan Univ Hosp, Dept Internal Med, Taipei 10002, Taiwan

[5] Natl Taiwan Univ, Coll Med, Taipei 10002, Taiwan

[6] Natl Taiwan Univ, Grad Inst Clin Med, Taipei 10002, Taiwan

来源：

LAB ON A CHIP | 2011年 / 11卷 / 10期

关键词：

STROMAL FIBROBLASTS; TUMOR-CELLS; MYOFIBROBLAST; MACROPHAGES; GROWTH; INVASION; PROMOTE;

D O I：

10.1039/c1lc20090a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We use a microfluidic cell culture chip equipped with pneumatic microvalves to analyze the paracrine loop between lung cancer cells and fibroblasts. In order to assess the cellular responses in the paracrine loop, we measure the migration speeds of cancer cells and the aspect ratios of fibroblasts which reflect the phenotype of myofibroblasts. With well-controlled interaction sequences between these two types of cells, we verify that the cytokines from cancer cells effectively stimulate the fibroblasts into myofibroblasts. The cytokines from myofibroblasts, rather than fibroblasts, increase the migration speeds of cancer cells. We confirm that the transforming growth factor-beta 1 (TGF-beta 1) is involved in the interaction between cancer cells and fibroblasts, and we also interrupt this paracrine loop in the cell culture chip by inhibiting the TGF-beta 1 receptors on fibroblasts.

引用

页码：1808 / 1814

页数：7

共 25 条

[1] Paracrine Signaling by Platelet-Derived Growth Factor-CC Promotes Tumor Growth by Recruitment of Cancer-Associated Fibroblasts [J].

Anderberg, Charlotte ;

Li, Hong ;

Fredriksson, Linda ;

Andrae, Johanna ;

Betsholtz, Christer ;

Li, Xuri ;

Eriksson, Ulf ;

Pietras, Kristian .

CANCER RESEARCH, 2009, 69 (01) :369-378

[2] Stromal fibroblasts in cancer initiation and progression [J].

Bhowmick, NA ;

Neilson, EG ;

Moses, HL .

NATURE, 2004, 432 (7015) :332-337

[3] TGF-β and cancer [J].

Bierie, B ;

Moses, HL .

CYTOKINE & GROWTH FACTOR REVIEWS, 2006, 17 (1-2) :29-40

[4] Direct-write laser micromachining and universal surface modification of PMMA for device development [J].

Cheng, JY ;

Wei, CW ;

Hsu, KH ;

Young, TH .

SENSORS AND ACTUATORS B-CHEMICAL, 2004, 99 (01) :186-196

[5] Selection of invasive and metastatic subpopulations from a human lung adenocarcinoma cell line [J].

Chu, YW ;

Yang, PC ;

Yang, SC ;

Shyu, YC ;

Hendrix, MJC ;

Wu, R ;

Wu, CW .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1997, 17 (03) :353-360

[6] Role of tissue stroma in cancer cell invasion [J].

De Wever, O ;

Mareel, M .

JOURNAL OF PATHOLOGY, 2003, 200 (04) :429-447

[7] The myofibroblast and its tumours [J].

Eyden, B. ;

Banerjee, S. S. ;

Shenjere, P. ;

Fisher, C. .

JOURNAL OF CLINICAL PATHOLOGY, 2009, 62 (03) :236-249

[8] Macrophages promote the invasion of breast carcinoma cells via a colony-stimulating factor-1/epidermal growth factor paracrine loop [J].

Goswami, S ;

Sahai, E ;

Wyckoff, JB ;

Cammer, N ;

Cox, D ;

Pixley, FJ ;

Stanley, ER ;

Segall, JE ;

Condeelis, JS .

CANCER RESEARCH, 2005, 65 (12) :5278-5283

[9] Development and multiplexed control of latching pneumatic valves using microfluidic logical structures [J].

Grover, WH ;

Ivester, RHC ;

Jensen, EC ;

Mathies, RA .

LAB ON A CHIP, 2006, 6 (05) :623-631

[10] Abrogation of TGF-β1-induced fibroblast-myofibroblast differentiation by histone deacetylase inhibition [J].

Guo, Weichao ;

Shan, Bin ;

Klingsberg, Ross C. ;

Qin, Xiangmei ;

Lasky, Joseph A. .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2009, 297 (05) :L864-L870

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