Concentration Effect on Quenching of Chlorophyll a Fluorescence by All-Trans-β-Carotene in Photosynthesis

被引:15
作者
Chen, Chen [1 ]
Gong, Nan [1 ]
Li, Zuowei [1 ]
Sun, Chenglin [2 ]
Men, Zhiwei [1 ]
机构
[1] Jilin Univ, Coll Phys, Coherent Light & Atom & Mol Spect Lab, Changchun 130012, Jilin, Peoples R China
[2] Jilin Univ, Coll Phys, Key Lab Phys & Technol Adv Batteries, Changchun 130012, Jilin, Peoples R China
基金
中国国家自然科学基金;
关键词
Chl-a; fluorescence; beta-Car; quenching; HARVESTING COMPLEX-II; ENERGY-TRANSFER; ANTENNA PROTEIN; SPECTROSCOPY; STATE; PHOTOPROTECTION; ABSORPTION; MOLECULE; SINGLET;
D O I
10.3390/molecules22101585
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Absorption, fluorescence spectra of chlorophyll a (Chl-a) and all-trans-beta-carotene (beta-Car) mixing solution are investigated in different polarity and polarizability solvents. The carotenoids regulate the energy flow in photosynthesis by interaction with chlorophyll, leading to an observable reduction of Chl-a fluorescence. The fluorescence red shifts with the increasing solvent polarizability. The energy transfer in the Chl-a and beta-Car system is proposed. The electron transfer should be dominant in quenching Chl-a fluorescence rather than the energy transfer in this system. Polar solvent with large polarizability shows high quenching efficiency. When dissolved in carbon tetrachloride, Chl-a presents red shift of absorption and blue shift of fluorescence spectra with increasing beta-Car concentration, which implies a Chl-a conformational change.
引用
收藏
页数:7
相关论文
共 21 条
  • [21] Tuning the Intrinsic Photophysical Properties of Chlorophyll a
    Wellman, Sydney M. J.
    Jockusch, Rebecca A.
    [J]. CHEMISTRY-A EUROPEAN JOURNAL, 2017, 23 (32) : 7728 - 7736