Characterization of acidic polysaccharides from the mollusks through acid hydrolysis

被引：25
作者：
Cao, Jiuling [1 ]
Wen, Chengrong [1 ,2 ,3 ]
Lu, Jiaojiao [1 ]
Teng, Nan [1 ]
Song, Shuang [1 ,2 ,3 ]
Zhu, Beiwei [1 ,2 ,3 ]
机构：
[1] Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China
[2] Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Dalian 116034, Peoples R China
[3] Dalian Polytech Univ, Natl & Local Joint Engn Lab Marine Bioact Polysac, Dalian 116034, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2015年 / 130卷
基金：
火炬计划; 中国国家自然科学基金;
关键词：
UACPs; Glycosaminoglycan; Disaccharide; HPLC-MS; Shellfish; FRUITING BODIES; HEPARAN-SULFATE; HYALURONIC-ACID; ANTICOAGULANT; GLYCOSAMINOGLYCANS; PURIFICATION; ABALONE;
D O I：
10.1016/j.carbpol.2015.05.006
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Uronic acid-containing polysaccharides (UACPs) including glycosaminoglycans (GAGs) exist widely in nature. Herein we propose an elegant methodology to identify UACPs by analyzing their disaccharides produced from the acid hydrolysis using HPLC-MSn upon 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. Based on the optimization of experimental conditions by the single factor experiment and orthogonal test design, the combination of 1.3 M TFA at 105 degrees C for 3 h is found to be the optimum. Subsequently, these conditions were applied to investigate the distribution of UACPs in 20 selected species of edible Bivalvia and Gastropoda. PMP-disaccharides derived from UACPs in mollusks were identified by comparing the retention time and mass spectra with those of the reference PMP-disaccharides from hyaluronic acid (HA), chondroitin sulfate (CS), heparin (HP), and AGSP with -->4)-GlcA(1-->2)-Man(1-->repeating units. The analysis reveals the prevalence of CS in the shellfishes as well as the HP, and existence of three non-GAG UACPs in 7 mollusks. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：268 / 274
页数：7
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