Hydrogen sulfide protects spinal cord and induces autophagy via miR-30c in a rat model of spinal cord ischemia-reperfusion injury

被引：66
作者：
Li, Lei [1 ]
Jiang, Hong-kun [2 ]
Li, Yun-peng [1 ]
Guo, Yan-ping [2 ]
机构：
[1] China Med Univ, Shengjing Hosp, Dept Orthoped, Shenyang 110004, Peoples R China
[2] China Med Univ, Affiliated Hosp 1, Dept Pediat, Shenyang 110001, Peoples R China
来源：
JOURNAL OF BIOMEDICAL SCIENCE | 2015年 / 22卷
关键词：
Autophagy; Beclin-1; miR-30c; Microtubule associated protein 1 light chain 3 (LC3); Oxygen glucose deprivation (OGD); ISCHEMIA/REPERFUSION INJURY; CELLS;
D O I：
10.1186/s12929-015-0135-1
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Background: Hydrogen sulfide (H2S), a novel gaseous mediator, has been recognized as an important neuromodulator and neuroprotective agent in the nervous system. The present study was undertaken to study the effects of exogenous H2S on ischemia/reperfusion (I/R) injury of spinal cord and the underlying mechanisms. Methods: The effects of exogenous H2S on I/R injury were examined by using assessment of hind motor function, spinal cord infarct zone by Triphenyltetrazolium chloride (TTC) staining. Autophagy was evaluated by expressions of Microtubule associated protein 1 light chain 3 (LC3) and Beclin-1 which were determined by using Quantitative Real-Time PCR and Western blotting, respectively. Results: Compared to I/R injury groups, H2S pretreatment had reduced spinal cord infarct zone, improved hind motor function in rats. Quantitative Real-Time PCR or Western blotting results showed that H2S pretreatment also downregulated miR-30c expression and upregulated Beclin-1 and LC3II expression in spinal cord. In vitro, miR-30c was showed to exert negative effect on Beclin-1 expression by targeting its 3'UTR in SY-SH-5Y cells treated with Oxygen, Glucose Deprivation (OGD). In rat model of I/R injury, pretreatment of pre-miR-30c or 3-MA (an inhibitor for autophagy) can abrogated spinal cord protective effect of H2S. Conclusion: H2S protects spinal cord and induces autophagy via miR-30c in a rat model of spinal cord hemia-reperfusion injury.
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页数：10
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