A new endophytic taxol- and baccatin III-producing fungus isolated from Taxus chinensis var. mairei

被引：22
|
作者：
Wang, Yechun [1 ]
Tang, Kexuan [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Sch Agr & Biol, Plant Biotechnol Res Ctr, Shanghai 200240, Peoples R China
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 72期
关键词：
Taxol; baccatin III; endophytic fungus; Didymostilbe sp; PESTALOTIOPSIS-MICROSPORA; PACLITAXEL; TAXANES; BREVIFOLIA; INDUCTION; YEW;
D O I：
10.5897/AJB11.876
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
120 endophytic fungi were isolated from the old inner bark of Taxus chinensis var. mairei and only a fungus was detected to produce Taxol and related taxanes in potato dextrose agar (PDA) medium. The presence of taxol and baccatin III was confirmed by high performance liquid chromatography combined with mass spectrometry (LC-MS) and competitive inhibition enzyme immunoassay (CIEIA). The fungal compound showed cytotoxic activity on liver cancer cell line BEL7402 in vitro. The strain was identified as one of Didymostilbe sp. (designated as DF110) according to its morphological characteristics. The isolation of such fungi provided a promising alternative approach to producing taxol in the near future.
引用
收藏
页码：16379 / 16386
页数：8
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