Cytogenetic Analysis of CpG-Oligonucleotide DSP30 plus Interleukin-2-Stimulated Canine B-Cell Lymphoma Cells Reveals the Loss of One X Chromosome as the Sole Abnormality

被引：3
|
作者：
Reimann-Berg, N. [1 ,2 ,3 ]
Escobar, H. Murua [1 ,2 ]
Kiefer, Y. [3 ]
Mischke, R. [1 ,2 ]
Willenbrock, S. [1 ,2 ,3 ]
Eberle, N. [1 ,2 ]
Nolte, I. [1 ,2 ]
Bullerdiek, J. [1 ,2 ,3 ]
机构：
[1] Univ Vet Med Hannover, Small Anim Clin, DE-30559 Hannover, Germany
[2] Univ Vet Med Hannover, Res Cluster REBIRTH, DE-30559 Hannover, Germany
[3] Univ Bremen, Ctr Human Genet, Bremen, Germany
来源：
CYTOGENETIC AND GENOME RESEARCH | 2011年 / 135卷 / 01期
关键词：
Animal model; Canine B-cell lymphoma; DSP30; IL-2; Monosomy X; PROSTATE-CANCER; MONOSOMY-X; ABERRATIONS; IL-2; DOGS; CLL;
D O I：
10.1159/000330126
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Human and canine lymphoid neoplasms are characterized by non-random cytogenetic abnormalities. However, due to the low mitotic activity of the B cells, cytogenetic analyses of B-cell lymphoid proliferations are difficult to perform. In the present study we stimulated canine B-cell lymphoma cells with the immunostimulatory CpG-oligonucleotide DSP30 in combination with interleukin-2 (IL-2) and obtained an adequate number of metaphases. Cytogenetic analyses revealed the loss of one X chromosome as the sole cytogenetic aberration. Chromosome analysis of the corresponding blood showed a normal female karyotype. Monosomy X as the sole clonal chromosomal abnormality is found in human hematopoietic malignancies as well, thus the dog may serve as a promising animal model. Copyright (C) 2011 S. Karger AG, Basel
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收藏
页码：79 / 82
页数：4
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