Direct patterning of mammalian cells onto porous tissue engineering substrates using agarose stamps

被引:103
作者
Stevens, MM
Mayer, M
Anderson, DG
Weibel, DB
Whitesides, GM
Langer, R [1 ]
机构
[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA
[2] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
关键词
hydroxyapatite; cell printing; osteoblast;
D O I
10.1016/j.biomaterials.2005.05.001
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
This paper describes simple, inexpensive, and potentially generic methodology for generating patterns of mammalian cells on porous scaffolds for tissue engineering using replica printing. Circular patterns (diameter: 200, 700, and 1000 mu m) of human osteoblasts were transferred directly from topographically patterned agarose stamps onto porous hydroxyapatite scaffolds or onto fibronectin-coated glass slides. The use of hydrogel stamps provided a "wet", biocompatible surface and maintained the viability of cells adsorbed on stamps during the patterning process. stamps inked once with suspensions of cells allowed the repeated patterning of substrates. Direct stamping of human osteoblasts (and, potentially other mammalian cells) can be used to control the size, spacing, and geometry of patterns of cells printed on porous tissue engineering substrates. This approach may find use in controlling the spatial invasion of scaffolds, promoting the hierarchical organization of cells, and in controlling cell-cell interactions as a step in preservation of phenotypes of cells. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:7636 / 7641
页数:6
相关论文
共 39 条
[1]   CELL CELL AND CELL MATRIX INTERACTIONS DIFFERENTIALLY REGULATE THE EXPRESSION OF HEPATIC AND CYTOSKELETAL GENES IN PRIMARY CULTURES OF RAT HEPATOCYTES [J].
BENZEEV, A ;
ROBINSON, GS ;
BUCHER, NLR ;
FARMER, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (07) :2161-2165
[2]   Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysine [J].
Branch, DW ;
Wheeler, BC ;
Brewer, GJ ;
Leckband, DE .
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2000, 47 (03) :290-300
[3]  
Brandow SL, 2002, CHEM-EUR J, V8, P5363, DOI 10.1002/1521-3765(20021202)8:23<5363::AID-CHEM5363>3.0.CO
[4]  
2-6
[5]   Hot embossing for micropatterned cell substrates [J].
Charest, JL ;
Bryant, LE ;
Garcia, AJ ;
King, WP .
BIOMATERIALS, 2004, 25 (19) :4767-4775
[6]   Geometric control of cell life and death [J].
Chen, CS ;
Mrksich, M ;
Huang, S ;
Whitesides, GM ;
Ingber, DE .
SCIENCE, 1997, 276 (5317) :1425-1428
[7]   Cellular micropatterns on biocompatible materials [J].
Folch, A ;
Toner, M .
BIOTECHNOLOGY PROGRESS, 1998, 14 (03) :388-392
[8]   THE USE OF NEURONAL NETWORKS ON MULTIELECTRODE ARRAYS AS BIOSENSORS [J].
GROSS, GW ;
RHOADES, BK ;
AZZAZY, HME ;
WU, MC .
BIOSENSORS & BIOELECTRONICS, 1995, 10 (6-7) :553-567
[9]   Micro- and nanotechnology via reaction-diffusion [J].
Grzybowski, BA ;
Bishop, KJM ;
Campbell, CJ ;
Fialkowski, M ;
Smoukov, SK .
SOFT MATTER, 2005, 1 (02) :114-128
[10]   Micropatterned surfaces modified with select peptides promote exclusive interactions with osteoblasts [J].
Hasenbein, ME ;
Andersen, TT ;
Bizios, R .
BIOMATERIALS, 2002, 23 (19) :3937-3942