Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients

被引：17
作者：
GallezHawkins, GM
Tegtmeier, BR
terVeer, A
Niland, JC
Forman, SJ
Zaia, JA
机构：
[1] CITY HOPE NATL MED CTR,DEPT PEDIAT,DUARTE,CA 91010
[2] CITY HOPE NATL MED CTR,DEPT INFECT DIS CLIN PATHOL,DUARTE,CA 91010
[3] CITY HOPE NATL MED CTR,DEPT BIOSTAT,DUARTE,CA 91010
[4] CITY HOPE NATL MED CTR,DEPT HEMATOL BONE MARROW TRANSPLANTAT,DUARTE,CA 91010
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 03期
关键词：
D O I：
10.1128/JCM.35.3.788-790.1997
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A plasma PCR test, using a nonradioactive PCR plate assay, was evaluated for detection of human cytomegalovirus reactivation. This assay was compared to Southern blotting and found to perform well. As a noncompetitive method of quantitation, it was similar to a competitive method for detecting the number of genome copies per milliliter of plasma in marrow transplant recipients. This is a technically simplified assay with potential for adaptation to automation.
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页码：788 / 790
页数：3
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