Metabolic profiling of epithelial ovarian cancer cell lines: evaluation of harvesting protocols for profiling using NMR spectroscopy

被引：13

作者：

Engskog, Mikael K. R. ^{[1
]}

Bjorklund, My ^{[2
]}

Haglof, Jakob ^{[1
]}

Arvidsson, Torbjorn ^{[1
,3
]}

Shoshan, Maria ^{[2
]}

Pettersson, Curt ^{[1
]}

机构：

[1] Uppsala Univ, Div Analyt Pharmaceut Chem, SE-75123 Uppsala, Sweden

[2] Karolinska Inst, Dept Oncol Pathol, Canc Ctr Karolinska R8 03, SE-17176 Stockholm, Sweden

[3] Med Prod Agcy, SE-75103 Uppsala, Sweden

来源：

BIOANALYSIS | 2015年 / 7卷 / 02期

关键词：

STEM; URINE; SERUM; HMDB;

D O I：

10.4155/bio.14.235

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Metabolic profiling represents a novel technology for analyzing tumor cells. Epithelial ovarian carcinoma has a low survival rate due to the development of aggressive and chemotherapy-resistant cells. A tailored and reliable protocol is presented for profiling of chemoresistant cells using the cell line SKOV3 and a multiresistant subline SKOV3R. Results: Harvesting protocols with cold methanol or MilliQ freeze/thaw cycles were compared. Increased reproducibility using MilliQ was evidenced. Importantly, both approaches resulted in similar profiles. Compared with parental SKOV3, the SKOV3R cells showed a significantly different profile. Conclusion: The MilliQ protocol is preferred owing to higher reproducibility and increased sample preparation options. The resulting metabolic profiles summarize metabolic alterations in chemoresistant cells consistent with a progressed and aggressive phenotype

引用

页码：157 / 166

页数：10

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