Enhanced abundance and activity of the chloroplast ATP synthase in rice through the overexpression of the AtpD subunit

被引:13
作者
Ermakova, Maria [1 ]
Heyno, Eiri [1 ]
Woodford, Russell [1 ]
Massey, Baxter [1 ]
Birke, Hannah [1 ]
von Caemmerer, Susanne [1 ]
机构
[1] Australian Natl Univ, Ctr Excellence Translat Photosynth, Res Sch Biol, Div Plant Sci, Canberra, ACT 2600, Australia
基金
澳大利亚研究理事会;
关键词
ATP synthase; CO2; assimilation; electron transport; photosynthesis; proton motive force; thylakoid membrane; ELECTRON-TRANSPORT; CARBOXYLASE OXYGENASE; CYTOCHROME B(6)/F; RUBISCO ACTIVITY; GAS-EXCHANGE; IN-VIVO; PHOTOSYNTHESIS; PROTEIN; ACTIVATION; DELTA;
D O I
10.1093/jxb/erac320
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Overexpression of the AtpD subunit of the chloroplast ATP synthase in rice increases the abundance and activity of the complex and stimulates photosynthetic electron transport at high light and high CO2. ATP, produced by the light reactions of photosynthesis, acts as the universal cellular energy cofactor fuelling all life processes. Chloroplast ATP synthase produces ATP using the proton motive force created by solar energy-driven thylakoid electron transport reactions. Here we investigate how increasing abundance of ATP synthase affects leaf photosynthesis and growth of rice, Oryza sativa variety Kitaake. We show that overexpression of AtpD, the nuclear-encoded subunit of the chloroplast ATP synthase, stimulates both abundance of the complex, confirmed by immunodetection of thylakoid complexes separated by Blue Native-PAGE, and ATP synthase activity, detected as higher proton conductivity of the thylakoid membrane. Plants with increased AtpD content had higher CO2 assimilation rates when a stepwise increase in CO2 partial pressure was imposed on leaves at high irradiance. Fitting of the CO2 response curves of assimilation revealed that plants overexpressing AtpD had a higher electron transport rate (J) at high CO2, despite having wild-type-like abundance of the cytochrome b(6)f complex. A higher maximum carboxylation rate (V-cmax) and lower cyclic electron flow detected in transgenic plants both pointed to an increased ATP production compared with wild-type plants. Our results present evidence that the activity of ATP synthase modulates the rate of electron transport at high CO2 and high irradiance.
引用
收藏
页码:6891 / 6901
页数:11
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