Antibacterial Activity of Silver Nanoparticles Synthesized by Aspergillus flavus and its Synergistic Effect with Antibiotics

被引:0
作者
Khleifat, Khaled [1 ,2 ]
Qaralleh, Haitham [3 ]
Al-Limoun, Mohammad [2 ]
Alqaraleh, Moath [4 ]
Abu Hajleh, Maha N. [5 ]
Al-Frouhk, Rahaf [2 ]
Al-Omari, Laila [6 ]
Al Buqain, Rula [7 ]
Dmour, Saif M. [8 ]
机构
[1] Al Ahliyya Amman Univ, Fac Allied Med Sci, Amman, Jordan
[2] Mutah Univ, Coll Sci, Biol Dept, Al Karak, Jordan
[3] Mutah Univ, Fac Sci, Dept Med Lab Sci, Al Karak, Jordan
[4] Al Ahliyya Amman Univ, Fac Pharm, Pharmacol & Diagnost Res Ctr PDRC, Amman 19328, Jordan
[5] Al Ahliyya Amman Univ, Fac Allied Med Sci, Pharmacol & Diagnost Res Ctr, Dept Cosmet Sci, Amman 19328, Jordan
[6] Zarqa Univ, Fac Allied Med Sci, Dept Med Lab, Zarqa, Jordan
[7] Univ Jordan, Cell Therapy Ctr, Amman, Jordan
[8] Al Hussein Bin Talal Univ, Prince Aisha Bint Al Hussein Fac Nursing & Hlth S, Dept Med Anal, Maan, Jordan
关键词
AgNPs; Aspergillus flavus; Antibacterial; Synergy; ANTIMICROBIAL ACTIVITY; STAPHYLOCOCCUS-AUREUS; ESCHERICHIA-COLI; GREEN SYNTHESIS; COMBINATION; EXTRACT;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microbial antibiotic resistance is rapidly increasing as a result of overuse or misuse of antibiotics, as well as a lack of new, effective antibiotics. Alternative antimicrobial treatments, such as nanoparticles, and their potential for stronger synergetic effect when paired with other active chemicals, could be a viable option. This study is prepared to estimate the antibacterial activity of silver nanoparticles (AgNPs) that have been synthesized using the biomass-free filtrate of Aspergillus flavus. The formation of AgNPs was reported by color changed to a dark brownish-black after 72 hours of incubation. The AgNPs surface plasmon resonance peak was indicated in the UV-Vis spectrum at 427 nm. The synthesis of AgNPs with a nanoparticle size of 10 to 35 nm was validated using transmission electron microscopy. The increase in folding area was calculated to detect the synergistic potential of the combined AgNPs with a broad range of conventional antibiotics. AgNPs have broad-spectrum activity against all strains tested. The most sensitive strain was Escherichia coli (11 mm), whereas the most resistant strain was Pseudomonas aeruginosa, as indicated by the lowest inhibition zone (7 mm). The lowest Minimum Inhibitory Concentration indicated was against K. pneumonia and Enterobacter cloacae (0.025 mg/ mL, each), followed by Staphylococcus epidermidis (0.05 mg/mL), E. coli and Shigella sp. (0.075 mg/mL, each), and then S. aureus (0.1 mg/mL). Notable synergy was reported between AgNPs and either ampicillin, erythromycin, ceftriaxone, vancomycin, azlocillin, or amoxicillin against S. aureus in the range between 29.3-fold to 8-fold. In addition, synergy was seen between AgNPs and either vancomycin, clindamycin, or erythromycin against P. aeruginosa (31.1-8.0-fold). Also, a maximum increase in IFA when erythromycin and vancomycin were synergized with AgNPs against E. cloacae was reported (IFA of 10.0 and 9.0, respectively). Similarly, AgNPs with either aztreonam or azlocillin against E. coli and amoxicillin, ciprofloxacin, or ceftriaxone against Shigella sp. caused an increase in the fold area of inhibition of between 5.3-3.7-fold. This result may have an advantage in encouraging the use of combined AgNPS with conventional antibiotics in treating infectious diseases caused by antibiotic-resistant bacteria.
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页数:14
相关论文
共 33 条
[1]   Synergistic and Antagonistic Effects of Metal Nanoparticles in Combination with Antibiotics Against Some Reference Strains of Pathogenic Microorganisms [J].
Abo-Shama, Usama H. ;
El-Gendy, Hanem ;
Mousa, Walid S. ;
Hamouda, Ragaa A. ;
Yousuf, Wesam E. ;
Hetta, Helal F. ;
Abdeen, Eman E. .
INFECTION AND DRUG RESISTANCE, 2020, 13 :351-362
[2]  
Al-Asoufi Ali, 2017, Pak J Biol Sci, V20, P179, DOI 10.3923/pjbs.2017.179.188
[3]  
ALrawashdeh I, 2019, Journal of Basic and Applied Research in Biomedicine, P89, DOI [10.51152/jbarbiomed.v5i2.40, DOI 10.51152/JBARBIOMED.V5I2.40]
[4]  
Althunibat OY, 2016, J PURE APPL MICROBIO, V10, P367
[5]   Green synthesis of anisotropic silver nanoparticles from the aqueous leaf extract of Dodonaea viscosa with their antibacterial and anticancer activities [J].
Anandan, M. ;
Poorani, G. ;
Boomi, P. ;
Varunkumar, K. ;
Anand, K. ;
Chuturgoon, A. Anil ;
Saravanan, M. ;
Prabu, H. Gurumallesh .
PROCESS BIOCHEMISTRY, 2019, 80 :80-88
[6]   Green synthesis of silver nanoparticles using Rheum palmatum root extract and their antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa [J].
Arokiyaraj, Selvaraj ;
Vincent, Savariar ;
Saravanan, Muthupandian ;
Lee, Yoonseok ;
Oh, Young Kyoon ;
Kim, Kyoung Hoon .
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, 2017, 45 (02) :372-379
[7]   Green chemical synthesis of gold nanoparticles by using Penicillium aculeatum and their scolicidal activity against hydatid cyst protoscolices of Echinococcus granulosus [J].
Barabadi, Hamed ;
Honary, Soheila ;
Mohammadi, Milad Ali ;
Ahmadpour, Ehsan ;
Rahimi, Mohammad Taghi ;
Alizadeh, Ahad ;
Naghibi, Farzaneh ;
Saravanan, Muthupandian .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2017, 24 (06) :5800-5810
[8]   Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity [J].
Bhakya, S. ;
Muthukrishnan, S. ;
Sukumaran, M. ;
Muthukumar, M. .
APPLIED NANOSCIENCE, 2016, 6 (05) :755-766
[9]   Fabrication of silver nanoparticles by Phoma glomerata and its combined effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus [J].
Birla, S. S. ;
Tiwari, V. V. ;
Gade, A. K. ;
Ingle, A. P. ;
Yadav, A. P. ;
Rai, M. K. .
LETTERS IN APPLIED MICROBIOLOGY, 2009, 48 (02) :173-179
[10]   SILVER NANOPARTICLES-DISK DIFFUSION TEST AGAINST Escherichia coli ISOLATES [J].
Cunha, Francisco Afranio ;
Maia, Kamila Rocha ;
Juca Mallman, Eduardo Jose ;
dos Santos Oliveira Cunha, Maria da Conceicao ;
Martins Maciel, Antonio Auberson ;
de Souza, Ieda Pereira ;
Menezes, Everardo Albuquerque ;
Almeida Fechine, Pierre Basilio .
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, 2016, 58