Detection and Characterization of Xanthomonas vasicola pv. vasculorum (Cobb 1894) comb. nov Causing Bacterial Leaf Streak of Corn in the United States

被引：42

作者：

Lang, J. M. ^{[1
]}

DuCharme, E. ^{[1
]}

Caballero, J. Ibarra ^{[1
]}

Luna, E. ^{[1
]}

Hartman, T. ^{[2
]}

Ortiz-Castro, M. ^{[1
]}

Korus, K. ^{[2
,3
]}

Rascoe, J. ^{[4
]}

Jackson-Ziems, T. A. ^{[2
]}

Broders, K. ^{[1
]}

Leach, J. E. ^{[1
]}

机构：

[1] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA

[2] Univ Nebraska Lincoln, Lincoln, NE 68583 USA

[3] Univ Florida, Alachua Cty Extens, Gainesville, FL 32609 USA

[4] USDA, Anim Plant Hlth Inspect Serv, Plant Protect & Quarantine, CPHST, Beltsville, MD 20705 USA

来源：

PHYTOPATHOLOGY | 2017年 / 107卷 / 11期

关键词：

FRAGMENT-LENGTH-POLYMORPHISM; PHYLOGENETIC ANALYSIS; VARIABLE-NUMBER; CAUSAL AGENT; ORYZAE; GENOMICS; TAXONOMY; SEQUENCE; MAIZE; RECLASSIFICATION;

D O I：

10.1094/PHYTO-05-17-0168-R

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Bacterial leaf streak of corn (Zea mays) recently reached epidemic levels in three corn-growing states, and has been detected in another six states in the central United States. Xanthomonas vasicola was identified as the causal agent of this disease. A multilocus sequence alignment of six housekeeping genes and comparison of average nucleotide identity from draft genome sequence were used to confirm phylogenetic relationships and classification of this bacteria relative to other X. vasicola strains. X. vasicola isolates from Nebraska and South Africa were highly virulent on corn and sugarcane and less virulent on sorghum but caused water-soaking symptoms that are typical of X. vasicola infection on the leaves of all three hosts. Based on host range and phylogenetic comparison, we propose the taxonomic designation of this organism to X. vasicola pv. vasculorum (Cobb 1894) comb. nov. Polymerase chain reaction-based diagnostic assays were developed that distinguish X. vasicola pv. vasculorum and X. vasicola pv. holcicola from each other and from other Xanthomonas spp.

引用

页码：1312 / 1321

页数：10

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