SPR-Based Detection of ASF Virus in Cells

被引：5

作者：

Capo, Alessandro ^{[1
,2
]}

Calabrese, Alessia ^{[1
,2
]}

Frant, Maciej ^{[3
]}

Walczak, Marek ^{[3
]}

Szczotka-Bochniarz, Anna ^{[3
]}

Manessis, Georgios ^{[4
]}

Bossis, Ioannis ^{[5
]}

Staiano, Maria ^{[1
]}

D'Auria, Sabato ^{[6
]}

Varriale, Antonio ^{[1
,2
]}

机构：

[1] CNR Italy, Inst Food Sci, I-83100 Avellino, Italy

[2] Univ Naples Federico II, CNR, URT ISA, Dept Biol, I-80126 Naples, Italy

[3] Natl Vet Res Inst NVRI, Dept Swine Dis, Al Partyzantow 57, PL-24100 Pulawy, Poland

[4] Agr Univ Athens AUA, Dept Anim Sci, Lab Anat & Physiol Farm Anim, Iera Odos 75 Str, Athens 11855, Greece

[5] Aristotle Univ Thessaloniki, Fac Agr Forestry & Nat Environm, Lab Anim Husb, Dept Anim Prod,Sch Agr, Thessaloniki 54124, Greece

[6] Natl Res Council Italy CNR, Dept Biol Agr & Food Sci, DISBA, Piazzale Aldo Moro 7, I-00185 Rome, Italy

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 13期

基金：

欧盟地平线“2020”;

关键词：

African swine fever virus (ASFV); detection; antibody; indirect ELISA test; surface plasmon resonance (SPR); AFRICAN-SWINE-FEVER; AMPLIFICATION; BIOSENSOR; PIG;

D O I：

10.3390/ijms23137463

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

African swine fever (ASF) is one of the most dangerous hemorrhagic infectious diseases that affect domestic and wild pigs. Currently, neither a vaccine nor effective treatments are available for this disease. As regards the degree of virulence, ASFV strains can be divided into high, moderate, or low virulence. The main detection methods are based on the use of the polymerase chain reaction (PCR). In order to prevent an uncontrolled spread of ASF, new on-site techniques that can enable the identification of an early-stage disease are needed. We have developed a specific immunological SPR-based assay for ASFV antigen detection directly in liquid samples. The developed assay allows us to detect the presence of ASFV at the dose of 10(3) HAD(50)/(mL).

引用

页数：16

共 45 条

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