Involvement of mitochondrial ferredoxin and Cox15p in hydroxylation of heme O

被引：121

作者：

Barros, MH

Carlson, CG

Glerum, DM

Tzagoloff, A ^{[1
]}

机构：

[1] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA

[2] Univ Alberta, Dept Med Genet, Edmonton, AB T6G 2S2, Canada

来源：

FEBS LETTERS | 2001年 / 492卷 / 1-2期

关键词：

COX15; heme O; hydroxylase; YAH1; cytochrome oxidase; mitochondrion;

D O I：

10.1016/S0014-5793(01)02249-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cox15p is essential for the biogenesis of cytochrome oxidase [Glerum et at., J, Biol. Chem, 272 (1997) 19088-19094]. We show here that cox15 mutants are blocked in heme A but not heme O biosynthesis. In Schizosaccharomyces pombe COX15 is fused to YAH1, the yeast gene for mitochondrial ferredoxin (adrenodoxin). A fusion of Cox15p and Yah1p in Saccharomyces cerevisiae rescued both cox15 and yah1 null mutants, This suggests that Yah1p functions in concert with Cox15p. We propose that Cox15p functions together with Yah1p and its putative reductase (Arh1p) in the hydroxylation of heme O. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：133 / +

页数：7

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