Serratia liquefaciens FG3 isolated from a metallophyte plant sheds light on the evolution and mechanisms of adaptive traits in extreme environments

被引：10

作者：

Caneschi, Washington Luiz ^{[1
]}

Sanchez, Angelica Bianchini ^{[1
]}

Felestrino, Erica Barbosa ^{[1
]}

de Carvalho Lemes, Camila Gracyelle ^{[1
]}

Cordeiro, Isabella Ferreira ^{[1
]}

Fonseca, Natasha Peixoto ^{[1
]}

Villa, Morghana Marina ^{[1
]}

Vieira, Izadora Tabuso ^{[1
]}

Goncalves Moraes, Lauro Angelo ^{[1
]}

Barbosa Assis, Renata de Almeida ^{[1
]}

do Carmo, Flavio Fonseca ^{[2
]}

Yoshino Kamino, Luciana Hiromi ^{[2
]}

Silva, Robson Soares ^{[3
]}

Ferro, Jesus Aparecido ^{[4
]}

Tiraboschi Ferro, Maria Ines ^{[4
]}

Ferreira, Rafael Marini ^{[4
]}

Santos, Vera Lucia ^{[5
]}

Mourao Silva, Ubiana de Cassia ^{[5
]}

Almeida, Nalvo Franco ^{[3
]}

Varani, Alessandro de Mello ^{[4
]}

Machado Garcia, Camila Carriao ^{[1
,6
]}

Setubal, Joao Carlos ^{[7
,8
]}

Moreira, Leandro Marcio ^{[1
,6
]}

机构：

[1] Univ Fed Ouro Preto, Nucleo Pesquisas Ciencias Biol NUPEB, Ouro Preto, MG, Brazil

[2] Inst Pristino, Belo Horizonte, MG, Brazil

[3] Univ Fed Mato Grosso do Sul, Fac Comp FACOM, Campo Grande, MS, Brazil

[4] UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet Jaboticabal, Dept Tecnol, Sao Paulo, SP, Brazil

[5] Univ Fed Minas Gerais, Dept Microbiol, Belo Horizonte, MG, Brazil

[6] Univ Fed Ouro Preto, ICEB, Dept Ciencias Biol DECBI, Ouro Preto, MG, Brazil

[7] Univ Sao Paulo, IQ, DB, Sao Paulo, SP, Brazil

[8] Virginia Tech, Biocomplex Inst, Blacksburg, VA USA

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

COMPARATIVE GENOMICS; TRANSFER-RNA; BACTERIAL; BIOSYNTHESIS; CELLULOSE; GENES; DNA; IDENTIFICATION; AGROBACTERIUM; SIDEROPHORES;

D O I：

10.1038/s41598-019-54601-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Serratia liquefaciens strain FG3 (SlFG3), isolated from the flower of Stachytarpheta glabra in the Brazilian ferruginous fields, has distinctive genomic, adaptive, and biotechnological potential. Herein, using a combination of genomics and molecular approaches, we unlocked the evolution of the adaptive traits acquired by S1FG3, which exhibits the second largest chromosome containing the largest conjugative plasmids described for Serratia. Comparative analysis revealed the presence of 18 genomic islands and 311 unique protein families involved in distinct adaptive features. S1FG3 has a diversified repertoire of genes associated with Nonribosomal peptides (NRPs/PKS), a complete and functional cluster related to cellulose synthesis, and an extensive and functional repertoire of oxidative metabolism genes. In addition, S1FG3 possesses a complete pathway related to protocatecuate and chloroaromatic degradation, and a complete repertoire of genes related to DNA repair and protection that includes mechanisms related to UV light tolerance, redox process resistance, and a laterally acquired capacity to protect DNA using phosphorothioation. These findings summarize that SlFG3 is well-adapted to different biotic and abiotic stress situations imposed by extreme conditions associated with ferruginous fields, unlocking the impact of the lateral gene transfer to adjust the genome for extreme environments, and providing insight into the evolution of prokaryotes.

引用

页数：16