Structural, Thermal, and Anti-inflammatory Properties of a Novel Pectic Polysaccharide from Alfalfa (Medicago sativa L.) Stem

被引:124
作者
Chen, Lei [1 ]
Liu, Jie [1 ]
Zhang, Yaqiong [1 ]
Dai, Bona [2 ]
An, Yuan [3 ]
Yu, Liangli [1 ,4 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Agr & Biol, Inst Food & Nutraceut Sci, Shanghai 200240, Peoples R China
[2] Shanghai Jiao Tong Univ, Instrumental Anal Ctr, Shanghai 200240, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China
[4] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA
基金
国家高技术研究发展计划(863计划); 中国博士后科学基金;
关键词
alfalfa pectic polysaccharide; rhamnogalacturonan I; thermal analysis; anti-inflammatory effect; CHEMICAL-COMPOSITION; C-13-NMR; ARABINAN; RHAMNOGALACTURONAN; CHROMATOGRAPHY; DECOMPOSITION; ELUCIDATION; DEGRADATION; SUBSTANCES; STABILITY;
D O I
10.1021/acs.jafc.5b00494
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
A pectic polysaccharide (APPS) was purified from the cold alkali extract of alfalfa stem and characterized to be a rhamnogalacturonan I (RG-I) type pectin with the molecular weight of 2.38 x 10(3) kDa and a radius of 123 nm. The primary structural analysis indicated that APPS composed of a -> 2)-alpha-L-Rhap-(1 -> 4)-alpha-D-GalpA-(1 -> backbone with 12% branching point at C-4 of Rhap forming side chains by l-arabinosyl and d-galactosyl oligosaccharide units. Transmission electron microscopy (TEM) analysis revealed a primary linear-shaped structure with a few branches in its assembly microstructures. The thermal decomposition evaluation revealed the stability of APPS with an apparent activation energy (E-a) of 226.5 kJ/mol and a pre-exponential factor (A) of 2.10 x 10(25)/s, whereas its primary degradation occurred in the temperature range from 215.6 to 328.0 degrees C. In addition, APPS showed significant anti-inflammatory effect against mRNA expressions of the pro-inflammatory cytokine genes, especially for IL-1 beta, suggesting its potential utilization in functional foods and dietary supplement products.
引用
收藏
页码:3219 / 3228
页数:10
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