Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica)

被引：0
|
作者：
Cingel, Aleksandar [1 ]
Vinterhalter, Branka [1 ]
Vinterhalter, Dragan [1 ]
Calic-Dragosavac, Dusica [1 ]
Smigocki, Ann [2 ]
Ninkovic, Slavica [1 ]
机构：
[1] Univ Belgrade, Inst Biol Res Sinisa Stankovic, Belgrade 11060, Serbia
[2] ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2010年 / 9卷 / 30期
关键词：
Agrobacterium tumefaciens; oryzacystatin; Solanum tuberosum L; PROTEINASE-INHIBITOR; PLANT-REGENERATION; SHOOT REGENERATION; MOLECULAR-CLONING; ORYZACYSTATIN-II; DIPLOID POTATO; EXPRESSION; RESISTANCE; GENE; LEAF;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragacevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler provided high shoot regeneration efficiency: 84 - 89% for Dragacevka cultivar and 60 - 68% for Jelica cultivar as compared to 76 - 86% for Desiree, the most frequently used cultivar in transformation experiments. PCR analysis of a small sample of putative transformants showed a nptII integration frequency of 90.9, 76.9 and 86.4% for Dragacevka, Jelica and Desiree, respectively. Regeneration and transformation efficiency was strongly genotype-dependent.
引用
收藏
页码：4644 / 4650
页数：7
相关论文
共 50 条
  • [1] In planta Agrobacterium-mediated transformation of L-Asparaginase gene into potato (Solanum tuberosum L.) cv. Agria: an efficient and novel method
    Amir Gharghi
    Arezoo Nazeri
    Ali Niazi
    Plant Biotechnology Reports, 2023, 17 : 149 - 158
  • [2] In planta Agrobacterium-mediated transformation of L-Asparaginase gene into potato (Solanum tuberosum L.) cv. Agria: an efficient and novel method
    Gharghi, Amir
    Nazeri, Arezoo
    Niazi, Ali
    PLANT BIOTECHNOLOGY REPORTS, 2023, 17 (02) : 149 - 158
  • [3] The efficient regeneration of the potato (Solanum tuberosum L.) cv. Igor in vitro
    Zel, J
    Medved, MM
    PHYTON-ANNALES REI BOTANICAE, 1999, 39 (03) : 277 - 282
  • [4] AGROBACTERIUM-MEDIATED TRANSFORMATION OF SOLANUM-TUBEROSUM L CV RUSSET BURBANK
    NEWELL, CA
    ROZMAN, R
    HINCHEE, MA
    LAWSON, EC
    HALEY, L
    SANDERS, P
    KANIEWSKI, W
    TUMER, NE
    HORSCH, RB
    FRALEY, RT
    PLANT CELL REPORTS, 1991, 10 (01) : 30 - 34
  • [5] Regeneration and Agrobacterium-mediated transformation studies in carnation (Dianthus caryophyllus L. cv. Turbo)
    Arici, S. E.
    Koc, N. K.
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2009, 8 (22): : 6094 - 6100
  • [6] Agrobacterium-mediated transformation of potato cv. Mnandi for resistance to the potato tuber moth (Phthorimaea operculella)
    Veale, M. A.
    Slabbert, M. M.
    Van Emmenes, L.
    SOUTH AFRICAN JOURNAL OF BOTANY, 2012, 80 : 67 - 74
  • [7] Agrobacterium-mediated transformation of indica rice cv. ADT 43
    A. Karthikeyan
    J. Shilpha
    S. Karutha Pandian
    M. Ramesh
    Plant Cell, Tissue and Organ Culture (PCTOC), 2012, 109 : 153 - 165
  • [8] Mixoploidy and chimeric structures in somaclones of potato (Solanum tuberosum L.) cv. Bintje
    Jelenic, S
    Berljak, J
    Papes, D
    Jelaska, S
    FOOD TECHNOLOGY AND BIOTECHNOLOGY, 2001, 39 (01) : 13 - 17
  • [9] Agrobacterium-mediated transformation of Java']Javanica rice cv. Rojolele
    Rachmawati, D
    Hosaka, T
    Inoue, E
    Anza, H
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2004, 68 (06) : 1193 - 1200
  • [10] Agrobacterium-mediated transformation of indica rice cv. ADT 43
    Karthikeyan, A.
    Shilpha, J.
    Pandian, S. Karutha
    Ramesh, M.
    PLANT CELL TISSUE AND ORGAN CULTURE, 2012, 109 (01) : 153 - 165
12345