Bioinformatics Analysis of the Expressed Sequence Tags from Rhizophora stylosa Griff. Genomic Library

This present study describes the bioinformatics analysis of 14 expressed sequence tags (ESTs) of salt tolerance genes in mangrove plant, Rhizophora stylosa Griff. on DDBJ/EMBL/GenBank as well as physical and chemical properties, similarity, phylogenetic, potential peptide, and subcellular localization. The physical and chemical properties of 14 ESTs showed diversity among the fragment genes. The DNA sequence among the ESTs from R. stylosa exhibited 49 % to 99 % among themselves. The target peptide value of chloroplast and mitochondrial varied from 0.094 to 0.544 and from 0.016 to 0.314, respectively, indicated it was possible to exist. These results suggested the importance of understanding the diversity and function of properties of the salt tolerance genes in R. stylosa genomic library. To confirm the relationship among the ESTs in R. stylosa, a phylogenetic tree was constructed. The phylogenetic tree shows that there are three clusters, the first branch contained one EST, the second branch consists of 11 genes, in which the majority ESTs resides and the last group comprised of two ESTs. The present study, therefore, suggested the diversity of ESTs salt tolerance genes form distinct clusters in the phylogenetic tree.