Conditioned medium from 3D culture system of stromal vascular fraction cells accelerates wound healing in diabetic rats

被引：20
作者：
Deng, Chengliang [1 ,2 ]
He, Yunfan [2 ]
Feng, Jingwei [2 ]
Dong, Ziqing [2 ]
Yao, Yao [2 ]
Lu, Feng [2 ]
机构：
[1] Zunyi Med Coll, Dept Plast Surg, Affiliated Hosp, Zunyi 563000, Guizhou, Peoples R China
[2] Southern Med Univ, Nanfang Hosp, Dept Plast Surg, Guangzhou 510515, Guangdong, Peoples R China
来源：
REGENERATIVE MEDICINE | 2019年 / 14卷 / 10期
关键词：
conditioned medium; extracellular matrix; fibroblast; keratinocyte; 3D culture; wound healing; MESENCHYMAL STEM-CELLS; HUMAN KERATINOCYTE; PROTEOMIC ANALYSIS; FLUID SHEAR; DIFFERENTIATION; MURINE; GROWTH; PROMOTES; DELIVERY; BIOLOGY;
D O I：
10.2217/rme-2018-0083
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Aim: We investigated the healing effects of conditioned medium (CM) derived from a physiological 3D culture system engineered to use an extracellular matrix/stromal vascular fraction (SVF) gel enriched for adipose on diabetic wounds in rats. This CM (Gel-CM) was compared with that from a 2D culture system that used SVF cells (SVF-CM). Materials & methods: Keratinocytes, fibroblasts and wounds were treated with Gel-CM and SVF-CM, and cytokine levels in the CM types were quantified. Results: Proliferation and migration of keratinocytes and fibroblasts were significantly higher after treatment with Gel-CM than with SVF-CM. Collagen secretion by fibroblasts and wound closure were highly stimulated by Gel-CM. Proteomic analyses revealed a higher concentration of growth factors in Gel-CM than in SVF-CM. Conclusion: Gel-CM is a promising therapeutic option for treating diabetic wounds.
引用
收藏
页码：925 / 937
页数：13
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