A strategy for rapid and efficient DNA sequencing by mass spectrometry

被引:109
作者
Koster, H
Tang, K
Fu, DJ
Braun, A
vandenBoom, D
Smith, CL
Cotter, RJ
Cantor, CR
机构
[1] SEQUENOM INC,BOSTON,MA 02110
[2] SEQUENOM INSTRUMENTS GMBH,D-22761 HAMBURG,GERMANY
[3] JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL,BALTIMORE,MD 21205
[4] BOSTON UNIV,CTR ADV BIOTECHNOL,BOSTON,MA 02215
关键词
DNA sequencing; solid phase; mass spectrometry;
D O I
10.1038/nbt0996-1123
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Two methods of solid-phase Sanger DNA sequencing followed by detection with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry are demonstrated. In one method, sequencing ladders generated on an immobilized synthetic template were resolved up to the 63-mer including the primer Detection sensivity and resolution were sufficient for sequence analysis in the given range, This approach is particularly suitable for comparative (diagnostic) DNA sequencing. A second method that has the potential for high throughput de novo DNA sequencing is also presented; it uses immobilized duplex probes with five-base single-stranded overhangs to capture an unknown DNA template serving as primers for Sanger DNA sequencing. The power of mass spectrometry is demonstrated not only by its very high speed, but also by its ability to identify sequences that are not readable using gel electrophoresis.
引用
收藏
页码:1123 / 1128
页数:6
相关论文
共 37 条
  • [1] STRINGS OF CONTIGUOUS MODIFIED PENTANUCLEOTIDES WITH INCREASED DNA-BINDING AFFINITY CAN BE USED FOR DNA-SEQUENCING BY PRIMER WALKING
    AZHIKINA, T
    VESELOVSKAYA, S
    MYASNIKOV, V
    POTAPOV, V
    ERMOLAYEVA, O
    SVERDLOV, E
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (24) : 11460 - 11462
  • [2] PROCEDURES FOR DETECTION OF DNA BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY USING A MODIFIED NAFION FILM SUBSTRATE
    BAI, J
    LIN, YH
    LIANG, XL
    ZHU, YD
    LUBMAN, DM
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1995, 9 (12) : 1172 - 1176
  • [3] DNA SEQUENCING - PRESENT LIMITATIONS AND PROSPECTS FOR THE FUTURE
    BARRELL, B
    [J]. FASEB JOURNAL, 1991, 5 (01) : 40 - 45
  • [4] ENHANCED DNA-SEQUENCING BY HYBRIDIZATION
    BROUDE, NE
    SANO, T
    SMITH, CL
    CANTOR, CR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) : 3072 - 3076
  • [5] HIGH-RESOLUTION MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT ANALYSIS OF SINGLE-STRANDED-DNA OF 27 TO 68 NUCLEOTIDES IN LENGTH
    CHRISTIAN, NP
    COLBY, SM
    GIVER, E
    HOUSTON, CT
    ARNOLD, RJ
    ELLINGTON, AD
    REILLY, JP
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1995, 9 (11) : 1061 - 1066
  • [6] MULTIPLEX DNA SEQUENCING
    CHURCH, GM
    KIEFFERHIGGINS, S
    [J]. SCIENCE, 1988, 240 (4849) : 185 - 188
  • [7] RAPID SEPARATION AND PURIFICATION OF OLIGONUCLEOTIDES BY HIGH-PERFORMANCE CAPILLARY GEL-ELECTROPHORESIS
    COHEN, AS
    NAJARIAN, DR
    PAULUS, A
    GUTTMAN, A
    SMITH, JA
    KARGER, BL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) : 9660 - 9663
  • [9] DNA-SEQUENCE DETERMINATION BY HYBRIDIZATION - A STRATEGY FOR EFFICIENT LARGE-SCALE SEQUENCING
    DRMANAC, R
    DRMANAC, S
    STREZOSKA, Z
    PAUNESKU, T
    LABAT, I
    ZEREMSKI, M
    SNODDY, J
    FUNKHOUSER, WK
    KOOP, B
    HOOD, L
    CRKVENJAKOV, R
    [J]. SCIENCE, 1993, 260 (5114) : 1649 - 1653
  • [10] A DNA-SEQUENCING STRATEGY THAT REQUIRES ONLY 5 BASES OF KNOWN TERMINAL SEQUENCE FOR PRIMING
    FU, DJ
    BROUDE, NE
    KOSTER, H
    SMITH, CL
    CANTOR, CR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (22) : 10162 - 10166