共 37 条
A strategy for rapid and efficient DNA sequencing by mass spectrometry
被引:109
作者:

Koster, H
论文数: 0 引用数: 0
h-index: 0
机构: SEQUENOM INC,BOSTON,MA 02110

Tang, K
论文数: 0 引用数: 0
h-index: 0
机构: SEQUENOM INC,BOSTON,MA 02110

Fu, DJ
论文数: 0 引用数: 0
h-index: 0
机构: SEQUENOM INC,BOSTON,MA 02110

Braun, A
论文数: 0 引用数: 0
h-index: 0
机构: SEQUENOM INC,BOSTON,MA 02110

vandenBoom, D
论文数: 0 引用数: 0
h-index: 0
机构: SEQUENOM INC,BOSTON,MA 02110

Smith, CL
论文数: 0 引用数: 0
h-index: 0
机构: SEQUENOM INC,BOSTON,MA 02110

Cotter, RJ
论文数: 0 引用数: 0
h-index: 0
机构: SEQUENOM INC,BOSTON,MA 02110

Cantor, CR
论文数: 0 引用数: 0
h-index: 0
机构: SEQUENOM INC,BOSTON,MA 02110
机构:
[1] SEQUENOM INC,BOSTON,MA 02110
[2] SEQUENOM INSTRUMENTS GMBH,D-22761 HAMBURG,GERMANY
[3] JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL,BALTIMORE,MD 21205
[4] BOSTON UNIV,CTR ADV BIOTECHNOL,BOSTON,MA 02215
关键词:
DNA sequencing;
solid phase;
mass spectrometry;
D O I:
10.1038/nbt0996-1123
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
Two methods of solid-phase Sanger DNA sequencing followed by detection with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry are demonstrated. In one method, sequencing ladders generated on an immobilized synthetic template were resolved up to the 63-mer including the primer Detection sensivity and resolution were sufficient for sequence analysis in the given range, This approach is particularly suitable for comparative (diagnostic) DNA sequencing. A second method that has the potential for high throughput de novo DNA sequencing is also presented; it uses immobilized duplex probes with five-base single-stranded overhangs to capture an unknown DNA template serving as primers for Sanger DNA sequencing. The power of mass spectrometry is demonstrated not only by its very high speed, but also by its ability to identify sequences that are not readable using gel electrophoresis.
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页码:1123 / 1128
页数:6
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