cDNA-AFLP analysis on transcripts associated with hydroxysafflor yellow A(HSYA) biosynthetic pathway in Carthamus tinctorius

被引：7

作者：

Feng, Na ^{[1
]}

Li, Yakui ^{[1
]}

Tang, Jie ^{[1
]}

Wang, Yan ^{[1
]}

Guo, Meili ^{[1
]}

机构：

[1] Second Mil Med Univ, Coll Pharm, Dept Pharmacognosy, Shanghai 200433, Peoples R China

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2010年 / 38卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Bulked segregant analysis (BSA); Carthamus tinctorius L; cDNA-AFLP; Evolution analysis; Hydroxysafflor yellow A (HSYA); Rapid amplification of cDNA ends (RACE); DISEASE-RESISTANCE; GENE-EXPRESSION; CANDIDATE GENES; FLAVONOIDS; CLONING;

D O I：

10.1016/j.bse.2010.09.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydroxysafflor yellow A (HSYA), an important active compound, is uniquely present in florets of Carthamus tinctorius. In current study, we applied cDNA amplified fragment length polymorphism (cDNA-AFLP) to screen genes expressed differently between plants with and without HSYA. One hundred and thirty-two primer combinations produced 6751 fragments, seven transcript-derived fragments (TDFs) showed consistent difference between two gene pools. An independent RT-PCR expression analysis validated the expression pattern for 3 TDFs (TDF-8, TDF-9 and TDF-27). The 3 TDFs were only transcripted in plants that contained HSYA, implying that they were associated with the formation of HSYA. In addition, a full length of TDF-8 was achieved, which termed as WV-prl. Bioinformatics analysis: showed that it carried a sequence highly homologous to the Broad bean wilt virus. Our study revealed that the three genes are involved in the process of response to some virus infection and functioning in HSYA production. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：971 / 980

页数：10

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