Effect of Different Extenders on Sperm Motility and Vitality in Goose Semen Cryopreservation

This study aimed to investigate the usability of different diluents containing 6% Dimethylformamide (DMF) for cryo-preservation of the semen of geese (Anser cygnoides). The diluents of Glucose (G), TrisGlucose (T), Lactated Ringer???s-Glucose (LG), and Lactated Ringer???s (L), all of which contained 6% DMF, were used as cryoprotectants. The researchers collected semen samples from four geese, twice a week over a four-week period, by means of abdominal massage; they then calculated how much sperm each goose ejaculated. Next, the semen samples were pooled and their spermatological parameters were determined. Their volume (4x (mL)), concentration (??108/mL), pH, motility (%), and vitality (%) rates were 0.31??0.01, 3.49??0.32, 7.13??1.06, 67.75??1.28, and 70.00??2.03, respectively. Then, these pooled semen samples were equally divided into four groups. Once they were frozen and thawed, the researchers discovered that the diluent L had the highest motility rate: 40.12% ?? 1.35. The motility rates of the other diluents were as follows: LG (28.25%?? 1.48), G (21.50% ?? 1.41), and T (5.12% ?? 0.83). Likewise, the vitality rates (%) of the diluents were as follows: L (41.93% ??1.87), LG (31.50%??1.88), G (29.43% ??1.45), and T (10.56%??1.34), respectively. Freezing and thawing appeared to lower each diluent???s vitality and motility rates. However, for the Lactated Ringer???s (L), this decrease was predictable. Therefore, Lactated Ringer???s diluent containing 6% DMF can be used in cryo-preservation of goose semen.