Smart hydrogel particles: Biomarker harvesting: One-step affinity purification, size exclusion, and protection against degradation

被引：150

作者：

Luchini, Alessandra ^{[1
,2
]}

Geho, David H. ^{[2
]}

Bishop, Barney ^{[3
]}

Tran, Duy ^{[2
]}

Xia, Cassandra ^{[2
]}

Dufour, Robert L. ^{[2
]}

Jones, Clinton D. ^{[2
]}

Espina, Virginia ^{[2
]}

Patanarut, Alexis ^{[3
]}

Zhou, Weidong ^{[2
]}

Ross, Mark M. ^{[2
]}

Tessitore, Alessandra ^{[2
]}

Petricoin, Emanuel F., III ^{[2
]}

Liotta, Lance A. ^{[2
]}

机构：

[1] NCI, CRO IRCCS, Aviano, Italy

[2] George Mason Univ, Ctr Appl Proteom & Mol Med, Manassas, VA 20110 USA

[3] George Mason Univ, Dept Chem & Biochem, Manassas, VA 20110 USA

来源：

NANO LETTERS | 2008年 / 8卷 / 01期

关键词：

D O I：

10.1021/nl072174l

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.

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收藏

页码：350 / 361

页数：12

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