Determination of glucosamine in impure chitin samples by high-performance liquid chromatography

被引：63
作者：
Zhu, XL
Cai, JB
Yang, J
Su, QD [1 ]
机构：
[1] Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
[2] Univ Sci & Technol China, Res Ctr Tobacco & Hlth, Hefei 230052, Peoples R China
来源：
CARBOHYDRATE RESEARCH | 2005年 / 340卷 / 10期
基金：
中国国家自然科学基金;
关键词：
glucosamine; chitin; derivatization; high-performance liquid chromatography;
D O I：
10.1016/j.carres.2005.01.045
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple, rapid, selective, and specific high-performance liquid chromatography (HPLC) method was developed to quantitate glucosamine, and its application for estimating purity of chitin was investigated. The chromatographic separation was achieved using a reversed-phase Q column, pre-column derivatization with 9-fluorenylmethoxycarbonyl chloride (Fmoc-Cl) and ultraviolet detection ( lambda = 254 nm). The mobile phase consisted of CH3CN and H2O. The optimum conditions of acid hydrolysis of chitin (concentration of HCl temperature, and heating time) was obtained by performing the orthogonal array design (OAD) procedure and the released glucosamine was determined by the above HPLC method. The accuracy of the method was checked by the standard addition technique. The method was found to be specific with good linearity, accuracy, precision, and well suited for quantitation of glucosamine and determination of the purity of chitin in biological materials and food products. (c) 2005 Elsevier Ltd. All rights reserved.
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收藏
页码：1732 / 1738
页数：7
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