RBD-Modified Bacterial Vesicles Elicited Potential Protective Immunity against SARS-CoV-2

被引：25

作者：

Yang, Zhongqian ^{[1
]}

Hua, Liangqun ^{[1
,2
]}

Yang, Mengli ^{[3
]}

Liu, Shu-Qun ^{[2
,4
,5
]}

Shen, Jianxin ^{[2
,4
,5
]}

Li, Weiran ^{[1
]}

Long, Qiong ^{[1
]}

Bai, Hongmei ^{[1
]}

Yang, Xu ^{[1
]}

Ren, Zhaoling ^{[6
,7
]}

Zheng, Xiao ^{[1
,2
]}

Sun, Wenjia ^{[1
]}

Ye, Chao ^{[1
]}

Li, Duo ^{[1
,8
]}

Zheng, Peng ^{[1
]}

He, Jinrong ^{[1
,7
]}

Chen, Yongjun ^{[1
]}

Huang, Weiwei ^{[1
]}

Peng, Xiaozhong ^{[3
,9
]}

Ma, Yanbing ^{[1
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biol, Lab Mol Immunol, Kunming, Yunnan, Peoples R China

[2] Yunnan Univ, Kunming, Yunnan, Peoples R China

[3] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biol, Natl Kunming High Level Biosafety Primate Res Ctr, Kunming, Yunnan, Peoples R China

[4] Yunnan Univ, State Key Lab Conservat & Utilizat Bioresources Y, Kunming, Yunnan, Peoples R China

[5] Yunnan Univ, Sch Life Sci, Kunming, Yunnan, Peoples R China

[6] Kunming Med Univ, Affiliated Hosp 2, Kunming, Yunnan, Peoples R China

[7] Kunming Med Univ, Kunming, Yunnan, Peoples R China

[8] Yunnan Prov Ctr Dis Control & Prevent, Dept Acute Infect Dis Control & Prevent, Kunming, Yunnan, Peoples R China

[9] Chinese Acad Med Sci, Peking Union Med Coll, State Key Lab Med Mol Biol,Neurosci Ctr,Sch Basic, Dept Mol Biol & Biochem,Inst Basic Med Sci,Med Pr, Beijing, Peoples R China

来源：

NANO LETTERS | 2021年 / 21卷 / 14期

基金：

中国国家自然科学基金;

关键词：

Bacterial vesicles; receptor binding domain; SARS-CoV-2; vaccine; COVID-19; OUTER-MEMBRANE VESICLES; CELLULAR-IMMUNITY; VACCINES; MATURATION;

D O I：

10.1021/acs.nanolett.1c00680

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The disease caused by SARS-CoV-2 infection threatens human health. In this study, we used high-pressure homogenization technology not only to efficiently drive the bacterial membrane to produce artificial vesicles but also to force the fusion protein ClyA-receptor binding domain (RBD) to pass through gaps in the bacterial membrane to increase the contact between ClyA-RBD and the membrane. Therefore, the load of ClyA-RBD on the membrane is substantially increased. Using this technology, we constructed a "ring-like" bacterial biomimetic vesicle (BBV) loaded with polymerized RBD (RBD-BBV). RBD-BBVs injected subcutaneously can accumulate in lymph nodes, promote antigen uptake and processing, and elicit SARS-CoV-2-specific humoral and cellular immune responses in mice. In conclusion, we evaluated the potential of this novel bacterial vesicle as a vaccine delivery system and provided a new idea for the development of SARS-CoV-2 vaccines.

引用

页码：5920 / 5930

页数：11

共 45 条

[1] Npro fusion technology to produce proteins with authentic N termini in E-coli [J].

Achmueller, Clemens ;

Kaar, Waltraud ;

Ahrer, Karin ;

Wechner, Philipp ;

Hahn, Rainer ;

Werther, Florian ;

Schmidinger, Hannes ;

Cserjan-Puschmann, Monika ;

Clementschitsch, Franz ;

Striedner, Gerald ;

Bayer, Karl ;

Jungbauer, Alois ;

Auer, Bernhard .

NATURE METHODS, 2007, 4 (12) :1037-1043

[2] Recombinant protein folding and misfolding in Escherichia coli [J].

Baneyx, F ;

Mujacic, M .

NATURE BIOTECHNOLOGY, 2004, 22 (11) :1399-1408

[3] The assembly dynamics of the cytolytic pore toxin ClyA [J].

Benke, Stephan ;

Roderer, Daniel ;

Wunderlich, Bengt ;

Nettels, Daniel ;

Glockshuber, Rudi ;

Schuler, Benjamin .

NATURE COMMUNICATIONS, 2015, 6

[4] Outer membrane vesicles displaying engineered glycotopes elicit protective antibodies [J].

Chen, Linxiao ;

Valentine, Jenny L. ;

Huang, Chung-Jr ;

Endicott, Christine E. ;

Moeller, Tyler D. ;

Rasmussen, Jed A. ;

Fletcher, Joshua R. ;

Boll, Joseph M. ;

Rosenthal, Joseph A. ;

Dobruchowska, Justyna ;

Wang, Zhirui ;

Heiss, Christian ;

Azadi, Parastoo ;

Putnam, David ;

Trent, M. Stephen ;

Jones, Bradley D. ;

DeLisa, Matthew P. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (26) :E3609-E3618

[5] A Hybrid Eukaryotic-Prokaryotic Nanoplatform with Photothermal Modality for Enhanced Antitumor Vaccination [J].

Chen, Qi ;

Huang, Guojun ;

Wu, Wangteng ;

Wang, Jianwei ;

Hu, Jiawei ;

Mao, Jianming ;

Chu, Paul K. ;

Bai, Hongzhen ;

Tang, Guping .

ADVANCED MATERIALS, 2020, 32 (16)

[6] DNA array and biological characterization of the impact of the maturation status of mouse dendritic cells on their phenotype and antitumor vaccination efficacy [J].

Chen, Z ;

Dehm, S ;

Bonham, K ;

Kamencic, H ;

Juurlink, B ;

Zhang, XS ;

Gordon, JR ;

Xiang, J .

CELLULAR IMMUNOLOGY, 2001, 214 (01) :60-71

[7] A Universal Design of Betacoronavirus Vaccines against COVID-19, MERS, and SARS [J].

Dai, Lianpan ;

Zheng, Tianyi ;

Xu, Kun ;

Han, Yuxuan ;

Xu, Lili ;

Huang, Enqi ;

An, Yaling ;

Cheng, Yingjie ;

Li, Shihua ;

Liu, Mei ;

Yang, Mi ;

Li, Yan ;

Cheng, Huijun ;

Yuan, Yuan ;

Zhang, Wei ;

Ke, Changwen ;

Wong, Gary ;

Qi, Jianxun ;

Qin, Chuan ;

Yan, Jinghua ;

Gao, George F. .

CELL, 2020, 182 (03) :722-+

[8] Immunostimulatory Potential of MoS2 Nanosheets: Enhancing Dendritic Cell Maturation, Migration and T Cell Elicitation [J].

Deng, Lei ;

Pan, Xiaoli ;

Zhang, Yulong ;

Sun, Sujing ;

Lv, Liping ;

Gao, Lei ;

Ma, Ping ;

Ai, Huisheng ;

Zhou, Qianqian ;

Wang, Xiaohui ;

Zhan, Linsheng .

INTERNATIONAL JOURNAL OF NANOMEDICINE, 2020, 15 :2971-2986

[9] Antibody-mediated immunity induced by engineered Escherichia coli OMVs carrying heterologous antigens in their lumen [J].

Fantappie, Laura ;

de Santis, Micaela ;

Chiarot, Emiliano ;

Carboni, Filippo ;

Bensi, Giuliano ;

Jousson, Olivier ;

Margarit, Immaculada ;

Grandi, Guido .

JOURNAL OF EXTRACELLULAR VESICLES, 2014, 3 (01)

[10] Simultaneous CD8+ T-Cell Immune Response against SARS-Cov-2 S, M, and N Induced by Endogenously Engineered Extracellular Vesicles in Both Spleen and Lungs [J].

Ferrantelli, Flavia ;

Chiozzini, Chiara ;

Manfredi, Francesco ;

Giovannelli, Andrea ;

Leone, Patrizia ;

Federico, Maurizio .

VACCINES, 2021, 9 (03) :1-19

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