In Vivo Toxicity Assessment of Laminarin Based Silver Nanoparticles from Turbinaria ornata in Adult Zebrafish (Danio rerio)

Silver nanoparticles synthesized using laminarin from Turbinaria ornata were subjected to acute toxicity in adult zebrafish. LC50 concentration of L-AgNPs and C-AgNPs at 96 h was noted to be 25.22 and 19.93 mg/L. To find out the fundamental toxicity mechanisms of L-AgNPs and C-AgNPs, the half of LC50 concentration 12.61 and 9.96 mg/L was exposed to adult zebrafish for 14 days respectively. There are no cytological changes in L-AgNPs whereas cytological changes were observed in gills, brain and liver tissues of C-AgNPs. The results concluded a plausible symbol for oxidative stress in zebrafish. The activities of antioxidant enzyme superoxide dismutase, catalase and glutathione peroxidase were decreased significantly in the C-AgNPs exposed while L-AgNPs showed no significant difference when compared to control. ICP-OES analysis of gills, liver and brain demonstrated that L-AgNPs and C-AgNPs revelation for 14 days resulted in enhanced silver uptake compared to fish treated with control are discussed in detail.