Lipolytic isoenzymes from Euphorbia latex

被引：31

作者：

Palocci, C

Soro, S

Cernia, E

Fiorillo, F

Belsito, CMA

Monacelli, B

Delle Monache, G

Pasqua, G

机构：

[1] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, Dept Plant Biol, I-00185 Rome, Italy

[3] Catholic Univ Sacro Cuore, Dept Chem, I-00100 Rome, Italy

来源：

PLANT SCIENCE | 2003年 / 165卷 / 03期

关键词：

plant lipases; euphorbiaceae; latex; terpenols; Euphorbia characias; Euphorbia wulfenii;

D O I：

10.1016/S0168-9452(03)00223-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity and substrate specificity of latex lipases from Euphorbia species (E characias, E wulfenii pinea, E myrsinites and E. dendroides) were investigated. High lipolytic activity was found only in E. characias and for the first time in E wulfenii latex. For both species the lipolytic activity on various triglycerides, and under different temperature and pH conditions, in both crude latex and in partially purified enzymes was quantified. Optimised extraction and purification methods permitted the recovery of the enzymatic fraction with high lipolytic activity. This fraction is probably constituted by a pool of different lipolytic enzymes. Finally, lipolytic activity was also measured for E characias and E wulfenii during vegetative and reproductive stages. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：577 / 582

页数：6

共 19 条

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