Automatic analysis of slides processed in the Comet assay

被引：46

作者：

Frieauff, W ^{[1
]}

Hartmann, A ^{[1
]}

Suter, W ^{[1
]}

机构：

[1] Novartis Pharma AG, Preclin Safety Toxicol Pathol, CH-4002 Basel, Switzerland

来源：

MUTAGENESIS | 2001年 / 16卷 / 02期

关键词：

D O I：

10.1093/mutage/16.2.133

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In recent years the Comet assay (or single cell gel electrophoresis assay) has been established as a rapid and sensitive method for the detection of DNA damage. For early genotoxicity screening of new chemical entities in industrial toxicology, the Comet assay is more and more used for assessment of the DNA damaging potential of a test compound. In order to increase compound screening throughput, we have established an image analysis system for fully automated measurement of microscope slides processed in the Comet assay. For the comparative investigation various cell types, such as V79 Chinese hamster cells, mouse lymphoma cells and human leukocytes, were treated with several test compounds, Using tail moment as the quantitative parameter for comet formation, we show a very high correlation between our automatic image analysis system and a commercially available, interactive system (Comet Assay II of Perceptive Instruments). The possibility of analyzing 50 samples within 1 day and the high reproducibility of results make automated image processing a powerful tool for automatic analysis of slides processed in the Comet assay.

引用

页码：133 / 137

页数：5

共 20 条

[1] Comet assay responses as indicators of carcinogen exposure [J].

Anderson, D ;

Yu, TW ;

McGregor, DB .

MUTAGENESIS, 1998, 13 (06) :539-555

[2] THE SINGLE-CELL GEL-ELECTROPHORESIS ASSAY FOR INDUCED DNA-DAMAGE (COMET ASSAY) - MEASUREMENT OF TAIL LENGTH AND MOMENT [J].

ASHBY, J ;

TINWELL, H ;

LEFEVRE, PA ;

BROWNE, MA .

MUTAGENESIS, 1995, 10 (02) :85-90

[3] A fast autofocus unit for fluorescence microscopy [J].

Bocker, W ;

Rolf, W ;

Muller, WU ;

Streffer, C .

PHYSICS IN MEDICINE AND BIOLOGY, 1997, 42 (10) :1981-1992

[4]

Bocker W, 1997, INT J RADIAT BIOL, V72, P449, DOI 10.1080/095530097143220

[5]

Böcker W, 1999, CYTOMETRY, V35, P134

[6] THE COMET ASSAY - A COMPREHENSIVE REVIEW [J].

FAIRBAIRN, DW ;

OLIVE, PL ;

ONEILL, KL .

MUTATION RESEARCH-REVIEWS IN GENETIC TOXICOLOGY, 1995, 339 (01) :37-59

[7] Automatic analysis of the in vitro micronucleus test on V79 cells [J].

Frieauff, W ;

Pötter-Locher, F ;

Cordier, A ;

Suter, W .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 1998, 413 (01) :57-68

[8] Exercise-induced DNA effects in human leukocytes are not accompanied by increased formation of 8-hydroxy-2'-deoxyguanosine or induction of micronuclei [J].

Hartmann, A ;

Pfuhler, S ;

Dennog, C ;

Germadnik, D ;

Pilger, A ;

Speit, G .

FREE RADICAL BIOLOGY AND MEDICINE, 1998, 24 (02) :245-251

[9] The contribution of cytotoxicity to DNA-effects in the single cell gel test (comet assay) [J].

Hartmann, A ;

Speit, G .

TOXICOLOGY LETTERS, 1997, 90 (2-3) :183-188

[10]

HARTMANN A, 2000, UNPUB ENV MOL MUTAGE

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