miR-126 modulates angiogenic growth parameters of peripheral blood endothelial progenitor cells

被引：29

作者：

Goerke, Sebastian M. ^{[1
]}

Kiefer, Lena S. ^{[1
]}

Stark, G. Bjoern ^{[1
]}

Simunovic, Filip ^{[1
]}

Finkenzeller, Guenter ^{[1
]}

机构：

[1] Univ Freiburg, Med Ctr, Dept Plast & Hand Surg, D-79106 Freiburg, Germany

来源：

BIOLOGICAL CHEMISTRY | 2015年 / 396卷 / 03期

关键词：

angiogenesis; migration; miR-126; peripheral blood EPC; proliferation; IN-VITRO ANGIOGENESIS; VASCULAR INTEGRITY; MICRORNA; BIOGENESIS; APOPTOSIS; ISCHEMIA; CLEAVAGE; MODEL;

D O I：

10.1515/hsz-2014-0259

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vascularization plays an important role in tissue engineering applications. It is known that implantation of differentiated endothelial cells or endothelial progenitor cells (EPCs) from cord blood (cbEPCs) gives rise to the formation of a complex functional neovasculature, whereas EPCs isolated from peripheral blood (pbEPCs) have a limited capability to form blood vessels upon implantation. MicroRNA-126 (miR-126) has been shown to have pro-angiogenic effects in vivo. In this study, we investigated whether modulation of miR-126 expression in pbEPCs may alter their angiogenic properties. Gain of function and loss of function experiments revealed that miR-126 has anti-angiogenic effects in pbEPCs. Overexpression of miR-126 resulted in decreased proliferation, migration, invasion and tube formation, while inhibition of miR-126 induced the opposite effects. However, modulation of miR-126 expression did not influence apoptotic susceptibility of pbEPCs. This study provides evidence that inhibition of miR-126 improves angio-genesis-related growth parameters in pbEPCs and may represent a therapeutic option to ameliorate the angiogenic and vasculogenic properties of pbEPCs.

引用

页码：245 / 252

页数：8

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