Identitication and characterization of the α-L-arabinofuranosidase B of Fusarium oxysporum f. sp dianthi

被引：10
作者：
Chacón-Martínez, CA
Anzola, JM
Rojas, A
Hernández, F
Junca, H
Ocampo, W
Del Portillo, P
机构：
[1] Corp CorpoGen, Dept Mol Biol, Bogota, Colombia
[2] German Res Ctr Biotechnol, GBF, Dept Environm Microbiol, D-38124 Braunschweig, Germany
来源：
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2004年 / 64卷 / 04期
关键词：
carnation; Fusarium wilt; phytopathogenic fungus; Hemicellulase; arabinase; alpha-L-arabinofuranosidase;
D O I：
10.1016/j.pmpp.2004.08.005
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The gene encoding alpha-L-Arabinofuranosidase B (abjB) from the phytopathogenic fungus Fusarhan oxysporum f. sp. dianthi (Fod) was identified, cloned, sequenced and heterologously expressed. AbfB consists in an intronless open reading frame of 1500-bp coding for a protein of 499 arninoacid residues. The partially purified fusion protein has an apparent molecular mass of approximately 58 kDa and exhibits a-L-arabinofuranosidase activity. The specific activity of the recombinant enzyme was 1.07 units mg(-1) protein. Optimal activity is attained at pH 4.0 and 50 degreesC. Interestingly, the abjB gene is actively transcribed in carnation plants infected with Fod. Its upregulation during the infection process suggests a possible role of this gene as virulence factor in the phytopathogenicity of this fungus. (C) 2004 Elsevier Ltd. All rights reserved.
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页码：201 / 208
页数：8
相关论文
共 36 条
[1]  
ALCONADA TM, 1994, FEMS MICROBIOL LETT, V118, P305, DOI 10.1016/0378-1097(94)90521-5
[2]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[3]   Cloning, disruption, and expression of two endo-beta 1,4-xylanase genes, XYL2 and XYL3, from Cochliobolus carbonum [J].
ApelBirkhold, PC ;
Walton, JD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (11) :4129-4135
[4]  
ARBELAEZ G, 1992, ACTA HORTIC, V300, P43
[5]   Genetic diversity in Fusarium oxysporum f sp dianthi and Fusarium redolens f sp dianthi [J].
Baayen, RP ;
vanDreven, F ;
Krijger, MC ;
Waalwijk, C .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 1997, 103 (05) :395-408
[6]  
Beckman C.H., 1987, NATURE WILT DIS PLAN
[7]   Gene structure prediction from consensus spliced alignment of multiple ESTs matching the same genomic locus [J].
Brendel, V ;
Xing, LQ ;
Zhu, W .
BIOINFORMATICS, 2004, 20 (07) :1157-1169
[8]  
CAETANOANOLLES G, 1993, PCR METH APPL, V3, P85
[9]   Characterization of an α-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum and its expression [J].
Carvallo, M ;
De Ioannes, P ;
Navarro, C ;
Chavez, R ;
Peirano, A ;
Bull, P ;
Eyzaguirre, J .
MYCOLOGICAL RESEARCH, 2003, 107 :388-394
[10]   Purification and characterization of an extracellular α-L-arabinofuranosidase from Fusarium oxysporum [J].
Christakopoulos, P ;
Katapodis, P ;
Hatzinikolaou, DG ;
Kekos, D ;
Macris, BJ .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2000, 87 (02) :127-133
1234