Nucleologenesis in Trypanosoma cruzi

被引:6
作者
Nepomuceno-Mejia, Tomas [1 ,3 ]
Lara-Martinez, Reyna [1 ]
Hernandez, Roberto [2 ]
de Lourdes Segura-Valdez, Maria [1 ]
Jimenez-Garcia, Luis F. [1 ]
机构
[1] Univ Nacl Autonoma Mexico, Lab Electron Microscopy, Fac Sci, Mexico City 04510, DF, Mexico
[2] Univ Nacl Autonoma Mexico, Biomed Res Inst, Dept Mol Biol & Biotechnol, Mexico City 04510, DF, Mexico
[3] CONACYT, Reg Ctr Publ Hlth Res, Tapachula, Chiapas, Mexico
关键词
Trypanosoma cruzi; nucleolus; nucleolar assembly; closed mitosis; pre-nucleolar bodies; NUCLEOLAR ORGANIZER REGIONS; PRENUCLEOLAR BODIES; LIVING CELLS; FINE-STRUCTURE; LIFE-CYCLE; MITOSIS; PROTEINS; DYNAMICS; EPIMASTIGOTES; TRANSCRIPTION;
D O I
10.1017/S1431927616000623
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Nucleolar assembly is a cellular event that requires the synthesis and processing of ribosomal RNA, in addition to the participation of pre-nucleolar bodies (PNBs) at the end of mitosis. Inmammals and plants, nucleolar biogenesis has been described in detail, but in unicellular eukaryotes it is a poorly understood process. In this study, we used light and electron microscopy cytochemical techniques to investigate the distribution of nucleolar components in the pathway of nucleolus rebuilding during closed cell division in epimastigotes of Trypanosoma cruzi, the etiologic agent of American trypanosomiasis. Silver impregnation specific for nucleolar organizer regions and an ethylenediaminetetraacetic acid regressive procedure to preferentially stain ribonucleoprotein revealed the conservation and dispersion of nucleolar material throughout the nucleoplasm during cell division. Furthermore, at the end of mitosis, the argyrophilic proteins were concentrated in the nucleolar organizer region. Unexpectedly, accumulation of nucleolar material in the form of PNBs was not visualized. We suggest that formation of the nucleolus in epimastigotes of T. cruzi occurs by a process that does not require the concentration of nucleolar material within intermediate nuclear bodies such as mammalian and plant PNBs.
引用
收藏
页码:621 / 629
页数:9
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