Ruthenium arene complexes with triphenylphosphane ligands: cytotoxicity towards pancreatic cancer cells, interaction with model proteins, and effect of ethacrynic acid substitution

被引：46

作者：

Biancalana, Lorenzo ^{[1
]}

Pratesi, Alessandro ^{[2
]}

Chiellini, Federica ^{[1
]}

Zacchini, Stefano ^{[3
]}

Funaioli, Tiziana ^{[1
]}

Gabbiani, Chiara ^{[1
]}

Marchetti, Fabio ^{[1
]}

机构：

[1] Univ Pisa, Dipartimento Chim & Chim Ind, Via G Moruzzi 13, I-56124 Pisa, Italy

[2] Univ Firenze, MetMed, Dipartimento Chim Ugo Schiff, Via Lastruccia 3, I-50019 Sesto Fiorentino, Italy

[3] Univ Bologna, Dipartimento Chim Ind Toso Montanari, Viale Risorgimento 4, I-40136 Bologna, Italy

来源：

NEW JOURNAL OF CHEMISTRY | 2017年 / 41卷 / 23期

关键词：

ORGANOMETALLIC RUTHENIUM(II)-ARENE COMPLEX; HALF-SANDWICH RUTHENIUM(II); ANTIPROLIFERATIVE ACTIVITY; ANTICANCER COMPLEXES; CONDUCTIVITY MEASUREMENTS; MASS-SPECTROMETRY; TRANSFERASE P1-1; OSMIUM COMPLEXES; METAL-COMPLEXES; CYTOCHROME-C;

D O I：

10.1039/c7nj02300f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The ruthenium-arene complexes [(eta(6)-p-cymene)RuCl2(kP-PPh2(4-C6H4CO2H))], 1, [(eta(6)-p-cymene)RuCl(kappa P-2, O-PPh2( 2-C6H4CO2))], 2, [(eta(6)-p-cymene)RuCl2(kP-PPh2(2-C6H4OCO-EA))], 3, and [(eta(6)-p-cymene)RuCl2(kP-PPh2(4C(6)H(4)CO(2)CH(2)CH(2)OCO-EA))], 4 (EA-CO2H = ethacrynic acid), were synthesized in good to high yields and characterized by analytical techniques, IR, UV-Vis and multinuclear NMR spectroscopy, and single crystal X-ray diffraction in the cases of 1 and 2. The unstable compounds [(eta(6)-arene)RuCl2(kP-PPh2(2C(6)H(4)CO(2)CH(2)CH(2)OCO-EA))] (arene = p-cymene, 5a; arene = C6H6, 5b) were obtained and identified in solution by NMR. Electrochemical and spectro-electrochemical experiments were performed in order to assess the redox behaviour of 1-4 in CH2Cl2. The in vitro cytotoxicity of 1-4 was determined on the human pancreatic cancer cell line BxPC3 and the mouse embryo fibroblast Balb/3T3 Clone A31 cell line, the latter acting as a model for normal cells. Furthermore, the interaction of 1, 3 and 4 with two model proteins was investigated by high resolution ESI-MS.

引用

页码：14574 / 14588

页数：15

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