HSPC117 Is the Essential Subunit of a Human tRNA Splicing Ligase Complex

被引：187

作者：

Popow, Johannes ^{[2
]}

Englert, Markus ^{[1
]}

Weitzer, Stefan ^{[2
]}

Schleiffer, Alexander ^{[3
]}

Mierzwa, Beata ^{[2
]}

Mechtler, Karl ^{[4
]}

Trowitzsch, Simon ^{[5
]}

Will, Cindy L. ^{[5
]}

Luehrmann, Reinhard ^{[5
]}

Soell, Dieter ^{[1
,6
]}

Martinez, Javier ^{[2
]}

机构：

[1] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

[2] Austrian Acad Sci IMBA, Inst Mol Biotechnol, A-1030 Vienna, Austria

[3] Res Inst Mol Pathol IMP IMBA, Bioinformat Dept, A-1030 Vienna, Austria

[4] Res Inst Mol Pathol IMP IMBA, Dept Prot Chem, A-1030 Vienna, Austria

[5] Max Planck Inst Biophys Chem, Dept Cellular Biochem, D-37077 Gottingen, Germany

[6] Yale Univ, Dept Chem, New Haven, CT 06520 USA

来源：

SCIENCE | 2011年 / 331卷 / 6018期

基金：

奥地利科学基金会;

关键词：

3'-TERMINAL PHOSPHATE CYCLASE; HELA-CELL EXTRACT; JUNCTION PHOSPHATE; FUNCTIONAL DOMAINS; LIGATION; PROTEIN; REVEALS; IDENTIFICATION; ENDONUCLEASE; PURIFICATION;

D O I：

10.1126/science.1197847

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Splicing of mammalian precursor transfer RNA (tRNA) molecules involves two enzymatic steps. First, intron removal by the tRNA splicing endonuclease generates separate 5' and 3' exons. In animals, the second step predominantly entails direct exon ligation by an elusive RNA ligase. Using activity-guided purification of tRNA ligase from HeLa cell extracts, we identified HSPC117, a member of the UPF0027 (RtcB) family, as the essential subunit of a tRNA ligase complex. RNA interference-mediated depletion of HSPC117 inhibited maturation of intron-containing pre-tRNA both in vitro and in living cells. The high sequence conservation of HSPC117/RtcB proteins is suggestive of RNA ligase roles of this protein family in various organisms.

引用

页码：760 / 764

页数：5

共 6 条

[1] Crystal structure of human archease, a key cofactor of tRNA splicing ligase complex
Duan, Shuyan
Gao, Wenqing
Chen, Zijun
Li, Zhengyang
Li, Suhua
Gan, Jianhua
Chen, Xiangjun
Li, Jixi
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2020, 122
[2] Homology model of the human tRNA splicing ligase RtcB
Nandy, Argha
Saenz-Mendez, Patricia
Gorman, Adrienne M.
Samali, Afshin
Eriksson, Leif A.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2017, 85 (11) : 1983 - 1993
[3] Molecular architecture of the human tRNA ligase complex
Kroupova, Alena
Ackle, Fabian
Asanovic, Igor
Weitzer, Stefan
Boneberg, Franziska M.
Faini, Marco
Leitner, Alexander
Chui, Alessia
Aebersold, Ruedi
Martinez, Javier
Jinek, Martin
ELIFE, 2021, 10
[4] Structural basis for pre-tRNA recognition and processing by the human tRNA splicing endonuclease complex
Hayne, Cassandra K.
Butay, Kevin John U.
Stewart, Zachary D.
Krahn, Juno M.
Perera, Lalith
Williams, Jason G.
Petrovitch, Robert M.
Deterding, Leesa J.
Matera, A. Gregory
Borgnia, Mario J.
Stanley, Robin E.
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2023, 30 (06) : 824 - +
[5] Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation
Loeff, Luuk
Kroupova, Alena
Asanovic, Igor
Boneberg, Franziska M.
Pfleiderer, Moritz M.
Riermeier, Luca
Leitner, Alexander
Ferdigg, Andre
Ackle, Fabian
Martinez, Javier
Jinek, Martin
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2025,
[6] UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcription
Turner, Declan L.
Fritzlar, Svenja
Sadeghipour, Sara
Barugahare, Adele A.
Russ, Brendan E.
Turner, Stephen J.
Mathias, Rommel A.
ISCIENCE, 2022, 25 (10)

← 1 →