Technique IRAP (inter retrotransposon amplified polymorphism) to study the genetic variability in Sclerotinia sclerotiorum populations

被引：1

作者：

Goldfarb, Miriam ^{[1
]}

Santana, Mateus Ferreira ^{[2
]}

Fernandes Salomao, Tania Maria ^{[3
]}

Scarpelli Pereira, Pedro Henrique ^{[4
]}

de Queiroz, Marisa Vieira ^{[5
]}

de Barros, Everaldo Goncalves ^{[6
]}

机构：

[1] Univ Fed Vicosa, Dept Gen Biol, Lab Mol Biol Plants, Appl Inst Biotechnol Agr BIOAGRO, BR-36570000 Vicosa, MG, Brazil

[2] Univ Fed Vicosa, Appl Inst Biotechnol Agr BIOAGRO, Dept Gen Biol, Mol Genet Lab Microorganism, BR-36570000 Vicosa, MG, Brazil

[3] Univ Fed Vicosa, Dept Gen Biol, Mol Biol Lab Insects, BR-36570000 Vicosa, MG, Brazil

[4] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, BR-05508220 Sao Paulo, SP, Brazil

[5] Univ Fed Vicosa, Appl Inst Biotechnol Agr BIOAGRO, Dept Microbiol, Mol Genet Lab Microorganism, BR-36570000 Vicosa, MG, Brazil

[6] Univ Catolica Brasilia, Dept Biotechnol, Genom Sci & Biotechnol Lab, BR-70790160 Brasilia, DF, Brazil

来源：

AUSTRALASIAN PLANT PATHOLOGY | 2016年 / 45卷 / 04期

关键词：

Bean plants; White mold; Transposons; Genetic variability; DIVERSITY;

D O I：

10.1007/s13313-016-0421-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Sclerotinia sclerotiorum, the causal agent of the white mold in dry beans, is responsible among other damages, for pronounced losses in bean cultivation. Therefore, there is an urgent need for greater research into the genetic variability of S. sclerotiorum. This study was done to evaluate the technical efficiency of IRAP (Inter Retrotransposon Amplified Polymorphism) to explore the genetic variability in this fungus. Primers were designing for the conserved regions of the long terminal repeats (LTRs) of the retrotransposons Copia-LTR_SS and Gypsy-LTR_SS. The primers were tested in the pathogen populations of S. sclerotiorum from Minas Gerais, Brazil. The estimated values of the high genetic variability indicate that the IRAP technique is a viable alternative and useful in population studies on the genetic variability of S. sclerotiorum.

引用

页码：357 / 360

页数：4

共 14 条

[1] Genomic Analysis of the Necrotrophic Fungal Pathogens Sclerotinia sclerotiorum and Botrytis cinerea [J].

Amselem, Joelle ;

Cuomo, Christina A. ;

van Kan, Jan A. L. ;

Viaud, Muriel ;

Benito, Ernesto P. ;

Couloux, Arnaud ;

Coutinho, Pedro M. ;

de Vries, Ronald P. ;

Dyer, Paul S. ;

Fillinger, Sabine ;

Fournier, Elisabeth ;

Gout, Lilian ;

Hahn, Matthias ;

Kohn, Linda M. ;

Lapalu, Nicolas ;

Plummer, Kim M. ;

Pradier, Jean-Marc ;

Quevillon, Emmanuel ;

Sharon, Amir ;

Simon, Adeline ;

ten Have, Arjen ;

Tudzynski, Bettina ;

Tudzynski, Paul ;

Wincker, Patrick ;

Andrew, Marion ;

Anthouard, Veronique ;

Beever, Ross E. ;

Beffa, Rolland ;

Benoit, Isabelle ;

Bouzid, Ourdia ;

Brault, Baptiste ;

Chen, Zehua ;

Choquer, Mathias ;

Collemare, Jerome ;

Cotton, Pascale ;

Danchin, Etienne G. ;

Da Silva, Corinne ;

Gautier, Angelique ;

Giraud, Corinne ;

Giraud, Tatiana ;

Gonzalez, Celedonio ;

Grossetete, Sandrine ;

Gueldener, Ulrich ;

Henrissat, Bernard ;

Howlett, Barbara J. ;

Kodira, Chinnappa ;

Kretschmer, Matthias ;

Lappartient, Anne ;

Leroch, Michaela ;

Levis, Caroline .

PLOS GENETICS, 2011, 7 (08)

[2]

Bianchini A, 2005, PHYTOPATHOLOGY MANUA, P333

[3]

Doyle JL., 1990, Focus, V12, P13, DOI DOI 10.2307/2419362

[4]

Goldfarb M, 2015, VARIABILIDADE GENETI

[5] DIVERSITY AND EVENNESS: A UNIFYING NOTATION AND ITS CONSEQUENCES [J].

HILL, MO .

ECOLOGY, 1973, 54 (02) :427-432

[6]

Ito F M, 2009, COMUNICADO TECNICO P

[7] Analysis of plant diversity with retrotransposon-based molecular markers [J].

Kalendar, R. ;

Flavell, A. J. ;

Ellis, T. H. N. ;

Sjakste, T. ;

Moisy, C. ;

Schulman, A. H. .

HEREDITY, 2011, 106 (04) :520-530

[8] IRAP and REMAP: two new retrotransposon-based DNA fingerprinting techniques [J].

Kalendar, R ;

Grob, T ;

Regina, M ;

Suoniemi, A ;

Schulman, A .

THEORETICAL AND APPLIED GENETICS, 1999, 98 (05) :704-711

[9]

Neergaard P., 1979, Seed pathology, VII.

[10] ANALYSIS OF GENE DIVERSITY IN SUBDIVIDED POPULATIONS [J].

NEI, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (12) :3321-3323

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