A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters

被引：21

作者：

Buehligen, Franziska ^{[1
]}

Lucas, Rico ^{[1
]}

Nikolausz, Marcell ^{[1
]}

Kleinsteuber, Sabine ^{[1
]}

机构：

[1] UFZ Helmholtz Ctr Environm Res, Dept Environm Microbiol, Permoserstr 15, D-04318 Leipzig, Germany

来源：

ANAEROBE | 2016年 / 39卷

关键词：

mcrA; Community fingerprinting; BstNl; Mwol; Methanogenic archaea; Biogas; 16S RIBOSOMAL-RNA; DIVERSITY; AMMONIA; GENES; RICH;

D O I：

10.1016/j.anaerobe.2016.03.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：114 / 116

页数：3

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