The potential of chitosan-based gels containing intervertebral disc cells for nucleus pulposus supplementation

被引:194
作者
Roughley, P [1 ]
Hoemann, C
DesRosiers, E
Mwale, F
Antoniou, J
Alini, M
机构
[1] McGill Univ, Genet Unit, Shriners Hosp Children, Montreal, PQ H3A 2T5, Canada
[2] McGill Univ, Dept Surg, Montreal, PQ H3A 2T5, Canada
[3] Ecole Polytech, Montreal, PQ H3C 3A7, Canada
[4] BioSyntech Canada Inc, Laval, PQ, Canada
[5] McGill Univ, Lady Davis Inst Med Res, Jewish Gen Hosp, Montreal, PQ H3A 2T5, Canada
[6] AO Res Inst, Davos, Switzerland
[7] McGill Univ, Orthopaed Res Lab, Montreal, PQ H3A 2T5, Canada
基金
加拿大健康研究院;
关键词
chitin/chitosan; cell encapsulation; hydrogel; intervertebral disc; nucleus pulposus; proteoglycan;
D O I
10.1016/j.biomaterials.2005.06.037
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The suitability of chitosan-based hydrogels as scaffolds for the encapsulation of intervertebral disc (IVD) cells and the accumulation of a functional extracellular matrix mimicking that of the nucleus pulposus (NP) was investigated. The specific hypothesis under study was that the cationic chitosan would form an ideal environment in which large quantities of newly synthesized anionic proteoglycan could be entrapped. Indeed, all the formulations of cell-seeded chitosan hydrogels, studied under in vitro culture conditions, showed that the majority of proteoglycan produced by encapsulated NP cells was retained within the gel rather than released into the culture medium. This was not always the case when annulus fibrosus cells were encapsulated, as unlike the nucleus cells the annulus cells often did not survive when cultured in chitosan. The results support the concept that chitosan may be a suitable scaffold for cell-based supplementation to help restore the function of the NP during the early stages of IVD degeneration. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:388 / 396
页数:9
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