Identification of small non-coding RNAs in the planarian Dugesia japonica via deep sequencing

被引：9

作者：

Qin, Yun-Fei ^{[1
]}

Zhao, Jin-Mei ^{[1
]}

Bao, Zhen-Xia ^{[1
]}

Zhu, Zhao-Yu ^{[1
]}

Mai, Jia ^{[1
]}

Huang, Yi-Bo ^{[1
]}

Li, Jian-Biao ^{[1
]}

Chen, Ge ^{[1
]}

Lu, Ping ^{[1
]}

Chen, San-Jun ^{[1
]}

Su, Lin-Lin ^{[1
]}

Fang, Hui-Min ^{[1
]}

Lu, Ji-Ke ^{[1
]}

Zhang, Yi-Zhe ^{[1
]}

Zhang, Shou-Tao ^{[1
]}

机构：

[1] Zhengzhou Univ, Dept Bioengn, Zhengzhou 450001, Henan, Peoples R China

来源：

GENOMICS | 2012年 / 99卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Deep sequencing; Dugesia japonica; Planarian; Regeneration; Small non-coding RNAs; BLASTEMA POLARITY; PIWI PROTEINS; STEM-CELLS; MICRORNAS; REGENERATION; NEOBLASTS; MECHANISM; DISCOVERY;

D O I：

10.1016/j.ygeno.2012.03.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Freshwater planarian flatworm possesses an extraordinary ability to regenerate lost body parts after amputation; it is perfect organism model in regeneration and stem cell biology. Recently, small RNAs have been an increasing concern and studied in many aspects, including regeneration and stem cell biology, among others. In the current study, the large-scale cloning and sequencing of sRNAs from the intact and regenerative planarian Dugesia japonica are reported. Sequence analysis shows that sRNAs between 18nt and 40nt are mainly microRNAs and piRNAs. In addition, 209 conserved miRNAs and 12 novel miRNAs are identified. Especially, a better screening target method, negative-correlation relationship of miRNAs and mRNA, is adopted to improve target prediction accuracy. Similar to miRNAs, a diverse population of piRNAs and changes in the two samples are also listed. The present study is the first to report on the important role of sRNAs during planarian Dugesia japonica regeneration. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：315 / 321

页数：7

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