Evaluation of Osmolality of Density Gradient for Human Islet Purification

被引:19
|
作者
Noguchi, Hirofumi [1 ,2 ,3 ]
Naziruddin, Bashoo [3 ,4 ,5 ]
Shimoda, Masayuki [6 ]
Fujita, Yasutaka [2 ]
Chujo, Daisuke [2 ]
Takita, Morihito [2 ]
Peng, Han [3 ]
Sugimoto, Koji [2 ]
Itoh, Takeshi [2 ]
Kobayashi, Naoya [1 ]
Onaca, Nicholas
Levy, Marlon F. [2 ,4 ,5 ]
Matsumoto, Shinichi [2 ]
机构
[1] Okayama Univ, Dept Surg Gastroenterol, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
[2] Baylor Res Inst, Baylor All St Med Ctr, Ft Worth, TX USA
[3] Baylor Univ, Inst Biomed Studies, Waco, TX 76798 USA
[4] Baylor Reg Transplant Inst, Dallas, TX USA
[5] Baylor Reg Transplant Inst, Ft Worth, TX USA
[6] Baylor Univ, Med Ctr, Baylor Heart & Vasc Inst, Div Cardiol,Dept Internal Med, Dallas, TX USA
关键词
Islet transplantation; Islet isolation; Iodixanol-Kyoto (IK) solution; Purification; Osmolality; PANCREAS PRESERVATION; DUCTAL INJECTION; GRAFT FUNCTION; TRANSPLANTATION; DONOR; OPTIMIZATION; INHIBITORS; PROTOCOL; YIELDS;
D O I
10.3727/096368911X605402
中图分类号
Q813 [细胞工程];
学科分类号
摘要
For pancreatic islet transplantation, the most common method of islet purification is density gradient centrifugation because of the differences in density between islets and acinar tissue. The density of islets/acinar tissue depends on several conditions, such as osmolality of purification solution. In this study, we evaluated the osmolality of iodixanol-controlled density gradients (400, 450, and 500 mOsm/kg) on the islet purification step. The density of the purification solutions was controlled by changing the volumetric ratio of iodixanol and the purification solutions (iodixanol-Kyoto solutions; IK solutions). The osmolality of density gradients was controlled by addition of 10x Hanks balanced salt solution (HBSS) solution. Density of both islets and acinar tissue increased relative to increase of the osmolality of purification solutions. There were no significant differences among the three groups on islet yield after density-adjusted purification and the rate of postpurification recovery. In vitro and in vivo assays suggest that the quality of islets was similar among the three groups. Our data suggest that efficacy of purification and quality of isolated islets is similar when the osmolality of purification solutions is between 400 and 500 mOsm/kg and density adjustment is applied. Since the density of islet and acinar tissue is changed according to osmolality, the density adjustment is important when using several osmolality solutions.
引用
收藏
页码:493 / 500
页数:8
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