Sequence analysis and molecular characterization of the Lactococcus lactis temperate bacteriophage BK5-T

被引：30
作者：
Mahanivong, C
Boyce, JD
Davidson, BE
Hillier, AJ
机构：
[1] Food Sci Australia, Werribee, Vic 3030, Australia
[2] Univ Melbourne, Russell Grimwade Sch Biochem & Mol Biol, Parkville, Vic 3010, Australia
[3] Univ Melbourne, Dept Food Sci & Agribusiness, Werribee, Vic 3030, Australia
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 08期
关键词：
D O I：
10.1128/AEM.67.8.3564-3576.2001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The Lactococcus lactis temperate bacteriophage BK5-T is one of twelve type phages that define L. lactis phage species. This paper describes the nucleotide sequence and analysis of a 21-kbp region of the BK5-T genome and completes the nucleotide sequence of the genome of this phage. The 40,003 -nucleotide linear genome encodes 63 open reading frames. Sequence runoff experiments showed that the cohesive ends of the BK5-T genome contained a 12-bp 3' single-stranded overhang with the sequence 5'-CACACACATAGG-3'. Two major BK5-T structural proteins, of approximately 30 and 20 kDa, were identified, and N-terminal sequence analysis determined that they were encoded by orf7 and orf12, respectively. A 169-bp fragment containing a 37-bp direct repeat and several smaller repeat sequences conferred resistance to BK5-T infection when introduced in trans to the host cell and is likely a part of the BK5-T origin of replication (ori).
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页码：3564 / 3576
页数：13
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