Imaging extracellular vesicles: current and emerging methods

被引：229

作者：

Chuo, Steven Ting-Yu ^{[1
]}

Chien, Jasper Che-Yung ^{[1
]}

Lai, Charles Pin-Kuang ^{[1
,2
,3
,4
]}

机构：

[1] Acad Sinica, Inst Atom & Mol Sci, 1 Roosevelt Rd,Sec 4, Taipei 10617, Taiwan

[2] Acad Sinica, Taiwan Int Grad Program, Chem Biol & Mol Biophys Program, Taipei, Taiwan

[3] Natl Taiwan Univ, Genome & Syst Biol Degree Program, Taipei, Taiwan

[4] Acad Sinica, Taipei, Taiwan

来源：

JOURNAL OF BIOMEDICAL SCIENCE | 2018年 / 25卷

关键词：

Extracellular vesicles exosomes; Microvesicles; Imaging; Biodistribution; Fluorescence; Bioluminescence; MRI; SPECT; Dyes; ATOMIC-FORCE MICROSCOPY; TRANSFERRIN RECEPTOR; MICROVESICLES; CELLS; BIODISTRIBUTION; EXOSOMES; TRACKING; PROTEIN; MIGRATION; MICRORNAS;

D O I：

10.1186/s12929-018-0494-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Extracellular vesicles (EVs) are lipid bilayer-enclosed nanoparticles released by cells. They range from 30nm to several micrometers in diameter, and ferry biological cargos such as proteins, lipids, RNAs and DNAs for local and distant intercellular communications. EVs have since been found to play a role in development, as well as in diseases including cancers. To elucidate the roles of EVs, researchers have established different methods to visualize and study their spatiotemporal properties. However, since EV are nanometer-sized, imaging them demands a full understanding of each labeling strategy to ensure accurate monitoring. This review covers current and emerging strategies for EV imaging for prospective studies.

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