Staphylococcus taiwanensis sp. nov., isolated from human blood

被引：5

作者：

Lin, Yu-Tzu ^{[1
]}

Hung, Wei-Chun ^{[2
]}

Wan, Tsai-Wen ^{[3
]}

Li, Hsin ^{[3
]}

Lee, Tai-Fen ^{[4
]}

Hsueh, Po-Ren ^{[5
,6
]}

Teng, Lee-Jene ^{[3
]}

机构：

[1] China Med Univ, Dept Med Lab Sci & Biotechnol, Taichung, Taiwan

[2] Kaohsiung Med Univ, Dept Microbiol & Immunol, Kaohsiung, Taiwan

[3] Natl Taiwan Univ Coll Med, Dept Clin Lab Sci & Med Biotechnol, Taipei, Taiwan

[4] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Dept Lab Med, Coll Med, Taipei, Taiwan

[5] China Med Univ, China Med Univ Hosp, Sch Med, Dept Lab Med, Taichung, Taiwan

[6] China Med Univ, China Med Univ Hosp, Sch Med, Dept Internal Med, Taichung, Taiwan

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2022年 / 72卷 / 02期

关键词：

novel species; Staphylococcus taiwanensis; bacteraemia; coagulase-negative staphylococci; methicillin resistance; COAGULASE-NEGATIVE STAPHYLOCOCCI; SUBSP NOV; IDENTIFICATION; HAEMOLYTICUS; GENOME; GENE; CLASSIFICATION; HSP60; ASSAY; SODA;

D O I：

10.1099/ijsem.0.005262

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel coagulase-negative Staphylococcus strain (NTUH-S172(T)) was isolated from human blood culture in Taiwan with preliminary identification of Staphylococcus saprophyticus. 16S rRNA gene analysis and multilocus sequence analysis (MLSA) showed that NTUH-S172( )(T)was most closely related to Staphylococcus haemolyticus. The average nucleotide identity and digital DNA-DNA hybridization values with the whole genome sequence were <95% and <70% when compared to the related species. Strain NTUH-S172(T) could be distinguished from S. haemolyticus by urease production and from Staphylococcus borealis by nitrate reduction. In addition, the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) spectrum of NTHU-S172(T) was significantly different from that of S. haemolyticus. which could be used in clinical identification. In conclusion, it is proposed that this isolate represents a novel species, named Staphylococcus taiwanensis sp. nov., with type strain NTUH-S172(T) (=BCRC 81315(T) =JCM 34726(T)).

引用

页数：7

共 40 条

[1] [Anonymous], 2020, EUR COM ANT SUSC TES
[2] Coagulase-Negative Staphylococci
Becker, Karsten
Heilmann, Christine
Peters, Georg
[J]. CLINICAL MICROBIOLOGY REVIEWS, 2014, 27 (04) : 870 - 926
[3] Chun J, 2018, INT J SYST EVOL MICR, V68, P461, DOI [10.1099/ijsem.0.002532, 10.1099/ijsem.0.002516]
[4] Clinical and Laboratory Standards Institute [CLSI], 2020, CLSI document M100-S15
[5] Staphylococcus haemolyticus - an emerging threat in the twilight of the antibiotics age
Czekaj, Tomasz
Ciszewski, Marcin
Szewczyk, Eligia M.
[J]. MICROBIOLOGY-SGM, 2015, 161 : 2061 - 2068
[6] Variability in SCCmecN1 spreading among injection drug users in Zurich, Switzerland
Ender, Miriam
Berger-Baechi, Brigitte
McCallum, Nadine
[J]. BMC MICROBIOLOGY, 2007, 7 (1)
[7] Methicillin-resistant staphylococcal bacteremia in patients with hematologic malignancies:: Clinical and microbiological retrospective comparative analysis of S-haemolyticus, S-epidermidis and S-aureus
Falcone, M
Micozzi, A
Pompeo, ME
Baiocchi, P
Fabi, F
Penni, A
Martino, P
Venditti, M
[J]. JOURNAL OF CHEMOTHERAPY, 2004, 16 (06) : 540 - 548
[8] Genetic classification and distinguishing of Staphylococcus species based on different partial gap, 16S rRNA, hsp60, rpoB, sodA, and tuf gene sequences
Ghebremedhin, B.
Layer, F.
Koenig, W.
Koenig, B.
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (03) : 1019 - 1025
[9] Staphylococcus saprophyticus subsp, bovis subsp nov, isolated from bovine nostrils
Hajek, V
Meugnier, H
Bes, M
Brun, Y
Fiedler, F
Chmela, Z
Lasne, Y
Fleurette, J
Freney, J
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1996, 46 (03): : 792 - 796
[10] Identification of Staphylococcus spp. by PCR-Restriction Fragment Length Polymorphism Analysis of dnaJ Gene
Hauschild, Tomasz
Stepanovic, Srdjan
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (12) : 3875 - 3879

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