Potential Antifibrotic and Angiostatic Impact of Idebenone, Carnosine and Vitamin E in Nano-Sized Titanium Dioxide-Induced Liver Injury

被引:30
作者
Abdelazim, Samy A. [1 ]
Darwish, Hebatallah A. [1 ]
Ali, Sanaa A. [2 ]
Rizk, Maha Z. [2 ]
Kadry, Mai O. [2 ]
机构
[1] Cairo Univ, Fac Pharm, Dept Biochem, Cairo, Egypt
[2] Natl Res Ctr, Therapeut Chem Dept, Dokki, Gizza, Egypt
关键词
Nano-titanium dioxide; In vitro; VEGF and western blot; PRIMARY BILIARY-CIRRHOSIS; DAMAGE; RATS; QUERCETIN;
D O I
10.1159/000374041
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Background/Aim: The present study investigated the in vitro and in vivo effects of individual and combined doses of idebenone, carnosine and vitamin E on ameliorating some of the biochemical indices of nano-sized titanium dioxide (n-TiO2) in mice liver. Methods: The in vitro cytotoxic effect of nano-sized anatase TiO2 (21 nm) on hepatic cell lines (HepG 2) was investigated. Additionally, n-TiO2 was orally administered (150 mg/kg/day) for 2 weeks, followed by a daily intragastric gavage of the aforementioned antioxidants for 1 month. Results: n-TiO2 induced significant cytotoxicity in hepatic cell lines and elevated the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic total antioxidant capacity (TAC) and nitrite/nitrate (NOx) levels. Meanwhile, glutathione-S-transferase (GST) activity was significantly reduced. Moreover, RT-PCR and western blot analysis showed that n-TiO2 significantly altered the mRNA and protein expressions of transforming growth factor-beta (TGF-beta 1) and Smad-2, as well as vascular endothelium growth factor (VEGF). Histopathological examination of hepatic tissue reinforced these results. Conclusion: Idebenone, carnosine and vitamin E ameliorated the deviated parameters with the combination regimen demonstrating the most pronounced effect. Oxidative stress, liver fibrosis and angiogenesis may be implicated in n-TiO2-induced liver toxicity. Copyright (C) 2015 S. Karger AG, Basel
引用
收藏
页码:2402 / 2411
页数:10
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