PYRENE INTERCALATING NUCLEIC ACIDS WITH A CARBON LINKER

被引：3
|
作者：
Ostergaard, Michael E. [1 ]
Wamberg, Michael C. [1 ]
Pedersen, Erik B. [1 ]
机构：
[1] Univ So Denmark, Dept Chem & Phys, Nucle Acid Ctr, DK-5230 Odense, Denmark
来源：
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2011年 / 30卷 / 03期
基金：
新加坡国家研究基金会;
关键词：
Intercalating nucleic acids; pyrene intercalators; INA; POINT MUTATIONS; DNA; EXCIMER; PROBE; HYBRIDIZATION;
D O I：
10.1080/15257770.2011.567202
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have synthesized a carbon linker analogue of INA (oligonucleotides containing insertions of 1-O-(1-pyrenylmethyl)glycerol). Thermal stability studies showed an increase in melting temperature in favor of the carbon linker analogue. We also synthesized a carbon linker analogue with two pyrenes geminally attached. Fluorescence studies of this intercalating nucleic acid with the pyrene moieties inserted as a bulge showed formation of an excimer band. When a mismatch was introduced at the site of the intercalator, an excimer band was formed for the destabilized duplexes whereas an exciplex band was observed when the stability of the duplex was retained.
引用
收藏
页码：210 / 226
页数：17
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