A highly specific far-red fluorescent probe for imaging endogenous peroxynitrite in the mitochondria of living cells

被引：102

作者：

Zhu, Baocun ^{[1
]}

Zhang, Meng ^{[1
]}

Wu, Liu ^{[1
]}

Zhao, Ziyang ^{[1
]}

Liu, Caiyun ^{[1
]}

Wang, Zuokai ^{[1
]}

Duan, Qingxia ^{[1
]}

Wang, Yawei ^{[1
]}

Jia, Pan ^{[1
]}

机构：

[1] Univ Jinan, Sch Resources & Environm, Shandong Prov Engn Technol Res Ctr Ecol Carbon Si, Jinan 250022, Shandong, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 257卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Peroxynitrite (ONOO-); Seminaphthorhodafluor; Far-red fluorescence; DRUG-INDUCED HEPATOTOXICITY; HYDROGEN-PEROXIDE; VISUALIZING PEROXYNITRITE; SELECTIVE DETECTION; BORONATE OXIDATION; FAST-RESPONSE; LIVE CELLS; CHEMOSENSORS; CHEMISTRY; BIOLOGY;

D O I：

10.1016/j.snb.2017.10.170

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of sensitive methods for imaging endogenous peroxynitrite (ONOO-) in the mitochondria of living cells is very important to disclose its diverse physiological and pathological functions. In this paper, we have described a simple, mitochondria-targeted far-red fluorescent probe Mito-PN for selectively detecting ONOO- even though in the presence of higher concentration (1 mM) other biologically important substances. The experimental results indicated that probe Mito-PN could quantitatively determine ONOO- in the range of 0.5-8 mu M with the detection limit of 17 nM. In addition, probe Mito-PN exhibits a fast response for ONOO- (<5 s), which is favorable for monitoring the highly reactive and short-lived ONOO- in the biological systems. The excellent sensing properties of probe Mito-PN enable its applications in the tracking of the fluctuations of endogenous ONOO- levels in the mitochondria of living cells. (c) 2017 Elsevier B.V. All rights reserved.

引用

页码：436 / 441

页数：6

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