Lysobacter lycopersici sp nov., isolated from tomato plant Solanum lycopersicum

A Gram-stain negative, aerobic, rod-shaped and non-spore-forming bacterium, CC-Bw-6(T) was isolated from chopped tomato stems. Analysis of the 16S rRNA gene sequence of the strain CC-Bw-6(T) showed its affiliation with the genus Lysobacter within the class Gammaproteobacteria. Strain CC-Bw-6(T) was found to be most closely related to Lysobacter panaciterrae KCTC 12601(T) (97.0 %) and Lysobacter daecheongensis KCTC 12600(T) (96.8 %), and showed lower similarity (< 96.5 %) to other Lysobacter species. DNA-DNA relatedness between strain CC-Bw-6(T) and L. panaciterrae KCTC 12601(T) was 10.8 %, the G+C content of the genomic DNA is 69.9 mol%. Strain CC-Bw-6(T) was determined to possess C-11:0 iso, C-11:0 iso 3OH, C-14:0 iso, C-15:0 anteiso, C-15:1 omega 5c, C-16:1 omega 5c, C-16:0, C-15:0 iso, C-16:0 iso, C-17:0 iso and C-16:0 10-methyl/C-17:1 iso omega 9c as predominant fatty acids. The major polar lipid profile consists of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified phospholipids. The predominant polyamine is spermidine, and ubiquinone-8 is the predominant respiratory quinone. Analysis of phenotypic, geno- and phylogenetic characteristics revealed a distinct taxonomic position of strain CC-Bw-6(T) with respect to other Lysobacter species. Based on the phylogenetic, chemotaxonomic and phenotypic data presented here, we propose a novel species with the name Lysobacter lycopersici sp. nov. The type strain is CC-Bw-6(T) (=BCRC 80612(T) = JCM 19164(T)).