Molecular identification of peptidoglycan recognition protein 5 and its functional characterization in innate immunity of large yellow croaker, Larimichthys crocea

被引:10
|
作者
Li, Qingfei [1 ,2 ]
Cui, Kun [1 ,2 ]
Xu, Dan [1 ,2 ]
Wu, Mengjiao [1 ,2 ]
Mai, Kangsen [1 ,2 ,3 ]
Ai, Qinghui [1 ,2 ,3 ]
机构
[1] Minist Agr, Key Lab Aquaculture Nutr & Feed, Qingdao, Peoples R China
[2] Ocean Univ China, Minist Educ, Coll Fisheries, Key Lab Mariculture, Qingdao, Peoples R China
[3] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao, Peoples R China
来源
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2021年 / 124卷
基金
中国国家自然科学基金;
关键词
Large yellow croaker; Peptidoglycan recognition protein 5; Phagocytosis; Amidase activity; Bacterial binding affinity; Anti-inflammatory activity; EXPRESSION; BACTERIA; LIPOPOLYSACCHARIDE; PHAGOCYTOSIS; DEFENSE; FISH;
D O I
10.1016/j.dci.2021.104130
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Fish peptidoglycan recognition proteins (PGRPs) play important roles in microbial recognition, and bacterial elimination. In the present study, a short-type PGRP from large yellow croaker, LcPGRP5 was cloned and its functions were characterized. LcPGRP5 gene encodes a protein containing conserved PGRP domain, but no signal peptide. Phylogenetic analysis shows that LcPGRP5 is clustered with other short PGRPs identified in other teleosts. LcPGRP5 is constitutively expressed in all tissues examined, with the highest expression being detected in the head kidney. Recombinant LcPGRP5 protein features amidase activity and bactericidal activity. Notably, LcPGRP5 could enhance the phagocytosis of the bacteria by large yellow croaker macrophage, with higher phagocytic capacity being observed in Staphylococcus aureus compared to Escherichia coli. Moreover, over expression of LcPGRP5 suppresses pro-inflammatory effects elicited by bacterial exposure in the macrophage cell line. Overall, the present results clearly indicate the important roles of LcPGRP5 played in the innate immune responses against bacterial infection.
引用
收藏
页数:10
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